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Intrinsically Fluorescent Silica Nanocontainers: A Promising Theranostic Platform

Published online by Cambridge University Press:  26 June 2013

Ana S. Rodrigues ,
Tânia Ribeiro ,
Fábio Fernandes ,
José Paulo S. Farinha  and
Carlos Baleizão
Ana S. Rodrigues
Affiliation:
Centro de Química-Física Molecular (CQFM) and Institute of Nanoscience and Nanotechnology (IN), Instituto Superior Técnico, 1049-001 Lisboa, Portugal
Tânia Ribeiro
Affiliation:
Centro de Química-Física Molecular (CQFM) and Institute of Nanoscience and Nanotechnology (IN), Instituto Superior Técnico, 1049-001 Lisboa, Portugal
Fábio Fernandes
Affiliation:
Centro de Química-Física Molecular (CQFM) and Institute of Nanoscience and Nanotechnology (IN), Instituto Superior Técnico, 1049-001 Lisboa, Portugal
José Paulo S. Farinha*
Affiliation:
Centro de Química-Física Molecular (CQFM) and Institute of Nanoscience and Nanotechnology (IN), Instituto Superior Técnico, 1049-001 Lisboa, Portugal
Carlos Baleizão*
Affiliation:
Centro de Química-Física Molecular (CQFM) and Institute of Nanoscience and Nanotechnology (IN), Instituto Superior Técnico, 1049-001 Lisboa, Portugal
*
*Corresponding author. E-mail: [email protected]
**Corresponding author. E-mail: [email protected]
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Abstract

In this paper we describe the preparation of fluorescent mesoporous silica nanoparticles (MSNs) for traceable drug delivery systems. The nanoparticles were prepared following a sol–gel procedure, incorporating a modified perylenediimide dye in the silica structure. Transmission electron microscopy and scanning electron microscopy show that the nanoparticles are monodispersed, with a spheroid shape and a raspberry-type surface morphology. The hybrid MSNs are robust, maintaining the mesoporous structure after template removal, with a pore diameter above 2 nm. A polymer shell was synthesized from the external surface of the hybrid nanoparticles by atom transfer radical polymerization, showing temperature-switchable collapsed/expanded conformation control. The fluorescent properties of the perylenediimide dye incorporated in the MSN pore walls are intact, and internalization in HEK293 cells shows that the nanoparticles are efficiently dispersed in the cytosol. These results show that the mesoporous fluorescent hybrid nanoparticles are an excellent platform for development of a traceable drug delivery system.

Keywords

mesoporous silica nanoparticlesperylenediimide derivativeslaser scanning confocal microscopyelectron microscopycore-shell nanoparticlescontrolled polymerizationPEG-acrylates
Type
Portuguese Society for Microscopy
Information
Microscopy and Microanalysis , Volume 19 , Issue 5 , October 2013 , pp. 1216 - 1221
Copyright
Copyright © Microscopy Society of America 2013 

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