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Influence of Blue Light on the Leaf Morphoanatomy of In Vitro Kalanchoe pinnata (Lamarck) Persoon (Crassulaceae)

Published online by Cambridge University Press:  30 July 2010

Marcos Vinicius Leal-Costa ,
Luana Beatriz dos Santos Nascimento ,
Nattacha dos Santos Moreira ,
Fernanda Reinert ,
Sônia Soares Costa ,
Celso Luiz Salgueiro Lage  and
Eliana Schwartz Tavares
Marcos Vinicius Leal-Costa*
Affiliation:
Universidade Federal do Rio de Janeiro, Centro de Ciências da Saúde, Instituto de Biologia, Departamento de Botânica, sala A1-104, Avenida Carlos Chagas Filho, Cidade Universitária, 21.941-902, Rio de Janeiro, Brazil Universidade Federal do Rio de Janeiro, Programa de pós-graduação em Biotecnologia Vegetal, Rio de Janeiro, Brazil
Luana Beatriz dos Santos Nascimento
Affiliation:
Universidade Federal do Rio de Janeiro, Centro de Ciências da Saúde, Instituto de Biologia, Departamento de Botânica, sala A1-104, Avenida Carlos Chagas Filho, Cidade Universitária, 21.941-902, Rio de Janeiro, Brazil
Nattacha dos Santos Moreira
Affiliation:
Universidade Federal do Rio de Janeiro, Centro de Ciências da Saúde, Instituto de Biologia, Departamento de Botânica, sala A1-104, Avenida Carlos Chagas Filho, Cidade Universitária, 21.941-902, Rio de Janeiro, Brazil
Fernanda Reinert
Affiliation:
Universidade Federal do Rio de Janeiro, Centro de Ciências da Saúde, Instituto de Biologia, Departamento de Botânica, sala A1-104, Avenida Carlos Chagas Filho, Cidade Universitária, 21.941-902, Rio de Janeiro, Brazil Universidade Federal do Rio de Janeiro, Programa de pós-graduação em Biotecnologia Vegetal, Rio de Janeiro, Brazil
Sônia Soares Costa
Affiliation:
Universidade Federal do Rio de Janeiro, Núcleo de Pesquisas de Produtos Naturais, Rio de Janeiro, Brazil
Celso Luiz Salgueiro Lage
Affiliation:
Universidade Federal do Rio de Janeiro, Instituto de Biofísica Carlos Chagas Filho, Rio de Janeiro, Brazil Universidade Federal do Rio de Janeiro, Programa de pós-graduação em Biotecnologia Vegetal, Rio de Janeiro, Brazil
Eliana Schwartz Tavares
Affiliation:
Universidade Federal do Rio de Janeiro, Centro de Ciências da Saúde, Instituto de Biologia, Departamento de Botânica, sala A1-104, Avenida Carlos Chagas Filho, Cidade Universitária, 21.941-902, Rio de Janeiro, Brazil
*
Corresponding author. E-mail: [email protected]
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Abstract

Kalanchoe pinnata (Lamarck) Persoon (Crassulaceae) (air plant, miracle leaf) is popularly used to treat gastrointestinal disorders and wounds. Recently, the species was tested to treat cutaneous leishmaniasis with successful results. This medicinal activity was associated with the phenolic fraction of the plant. Blue light induces biosynthesis of phenolic compounds and many changes in anatomical characteristics. We studied the effects of supplementary blue light on the leaf morphology of in vitro K. pinnata. Plants cultured under white light (W plants) only and white light plus blue light (WB plants) show petioles with plain-convex section, amphistomatic leaf blades with simple epidermis, homogeneous mesophyll with densely packed cells, and a single collateral vascular bundle in the midrib. W plants have longer branches, a larger number of nodes per branch, and smaller leaves, whereas WB plant leaves have a thicker upper epidermis and mesophyll. Leaf fresh weight and leaf dry weight were similar in both treatments. Phenolic idioblasts were observed in the plants supplemented with blue light, suggesting that blue light plays an important role in the biosynthesis of phenolic compounds in K. pinnata.

Keywords

blue lightCrassulaceaein vitro culture of medicinal plantsplant developmentKalanchoe pinnataleaf anatomy
Type
Biological Applications
Information
Microscopy and Microanalysis , Volume 16 , Issue 5 , October 2010 , pp. 576 - 582
Copyright
Copyright © Microscopy Society of America 2010

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