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In Situ Structural Analysis of Bacterial Nanomachines by Combining Cryo-FIB Milling, Cryo-ET and Sub-tomogram Analysis

Published online by Cambridge University Press:  30 July 2020

Yunjie Chang  and
Jun Liu
Yunjie Chang
Affiliation:
Department of Microbial Pathogenesis, Yale University School of Medicine, West Haven, Connecticut, United States Microbial Sciences Institute, Yale University, West Haven, Connecticut, United States
Jun Liu
Affiliation:
Department of Microbial Pathogenesis, Yale University School of Medicine, West Haven, Connecticut, United States Microbial Sciences Institute, Yale University, West Haven, Connecticut, United States

Abstract

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Type
The Promise of Cryo-Electron Tomography
Information
Microscopy and Microanalysis , Volume 26 , Supplement S2 , August 2020 , pp. 1992 - 1993
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Copyright
Copyright © Microscopy Society of America 2020

References

Mastronarde, D.N., Automated electron microscope tomography using robust prediction of specimen movements. Journal of Structural Biology, 2005. 152(1): p. 36-51.10.1016/j.jsb.2005.07.007CrossRefGoogle ScholarPubMed
Zheng, S.Q., et al. ., MotionCor2: anisotropic correction of beam-induced motion for improved cryo-electron microscopy. Nature Methods, 2017. 14: p. 331.10.1038/nmeth.4193CrossRefGoogle ScholarPubMed
Kremer, J.R., Mastronarde, D.N., and McIntosh, J.R., Computer Visualization of Three-Dimensional Image Data Using IMOD. Journal of Structural Biology, 1996. 116(1): p. 71-76.10.1006/jsbi.1996.0013CrossRefGoogle ScholarPubMed
Zhang, K., Gctf: Real-time CTF determination and correction. J Struct Biol, 2016. 193(1): p. 1-12.10.1016/j.jsb.2015.11.003CrossRefGoogle ScholarPubMed
Winkler, H., 3D reconstruction and processing of volumetric data in cryo-electron tomography. Journal of Structural Biology, 2007. 157(1): p. 126-137.10.1016/j.jsb.2006.07.014CrossRefGoogle ScholarPubMed
Winkler, H., et al. ., Tomographic subvolume alignment and subvolume classification applied to myosin V and SIV envelope spikes. Journal of Structural Biology, 2009. 165(2): p. 64-77.10.1016/j.jsb.2008.10.004CrossRefGoogle ScholarPubMed
Chang, Y., et al. ., Structural insights into flagellar stator-rotor interactions. Elife, 2019. 8.10.7554/eLife.48979CrossRefGoogle ScholarPubMed