Hostname: page-component-cd9895bd7-gbm5v Total loading time: 0 Render date: 2024-12-27T11:17:41.954Z Has data issue: false hasContentIssue false
  • English
  • Français

Immunohistochemical Identification and Electron Microscopic Study on Early Migrating Neural Crest Cells in the Chick Embryo

Published online by Cambridge University Press:  02 July 2020

M. Monteagudo de la Rosa ,
M. González-Santander Martínez ,
G. Martinez Cuadrado  and
R. González Santander
M. Monteagudo de la Rosa
Affiliation:
Departamento de Ciencias Morfológicas y Cirugîa. Facultad de Medicina. Universidad de Alcalá de Henares. E-28871. Madrid. Spain.
M. González-Santander Martínez
Affiliation:
Departamento de Ciencias Morfológicas y Cirugîa. Facultad de Medicina. Universidad de Alcalá de Henares. E-28871. Madrid. Spain.
G. Martinez Cuadrado
Affiliation:
Departamento de Ciencias Morfológicas y Cirugîa. Facultad de Medicina. Universidad de Alcalá de Henares. E-28871. Madrid. Spain.
R. González Santander
Affiliation:
Departamento de Ciencias Morfológicas y Cirugîa. Facultad de Medicina. Universidad de Alcalá de Henares. E-28871. Madrid. Spain.
Get access

Extract

Just after neural fold fusion to form the neural tube, neural crest cells detach from the neural crest, a transient structure located in the dorsal region of the neural tube. Neural crest cells migrate and differentiate into many structures and cells. But the underlying controls of this detachment and initiation of emigration are unknown. Neural crest cells are usually not morphologically distinct from the adjacent neural epithelium (neural tube) and epidermal ectoderm (epiblast) flanking them. We are combining morphological and immunohistochemical approaches to study neural crest cells in their early stage of detachment from the neural crest.

Hamburger and Hamilton (1951) stages 9 to 12 White Leghorn chick embryos. Fixation in 2.5% glutaraldehyde - 0.5% tanic acid and postfixation in 1% osmium tetroxide. Embryos contrasted in bloc using uranyl acetate and embedded in araldite. Semithin transversal sections stained with toluidine blue for light microscopy. Ultrathin sections contrasted with lead citrate.

Type
Developmental and Reproductive Biology
Information
Microscopy and Microanalysis , Volume 3 , Issue S2: Proceedings: Microscopy & Microanalysis '97, Microscopy Society of America 55th Annual Meeting, Microbeam Analysis Society 31st Annual Meeting, Histochemical Society 48th Annual Meeting, Cleveland, Ohio, August 10-14, 1997 , August 1997 , pp. 177 - 178
Copyright
Copyright © Microscopy Society of America 1997

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

1.Bronner-Fraser, M., FASEB J. (1994)8: 699.10.1096/fasebj.8.10.8050668CrossRefGoogle Scholar
2.Smith-Thomas, I.C.et al., Dev. Brain Res. (1994)81: 171.10.1016/0165-3806(94)90303-4CrossRefGoogle Scholar