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Immunogold Labeling for Igg, Immunoglobulin Light Chains and P-Component in Fibrillary and Immunotactoid Glomerular Nephritis

Published online by Cambridge University Press:  02 July 2020

S. Hearn ,
J. Walton ,
M. Moussa ,
Mark. Rieckenberg  and
K. Hutcheson
S. Hearn
Affiliation:
Departments of Pathology, St. Joseph's Health Centre and London Health Science Centre, London Ontario Canada, and Clinical Laboratories Thunder Bay Regional Hospital, Thunder Bay, Ontario.
J. Walton
Affiliation:
Departments of Pathology, St. Joseph's Health Centre and London Health Science Centre, London Ontario Canada, and Clinical Laboratories Thunder Bay Regional Hospital, Thunder Bay, Ontario.
M. Moussa
Affiliation:
Departments of Pathology, St. Joseph's Health Centre and London Health Science Centre, London Ontario Canada, and Clinical Laboratories Thunder Bay Regional Hospital, Thunder Bay, Ontario.
Mark. Rieckenberg
Affiliation:
Departments of Pathology, St. Joseph's Health Centre and London Health Science Centre, London Ontario Canada, and Clinical Laboratories Thunder Bay Regional Hospital, Thunder Bay, Ontario.
K. Hutcheson
Affiliation:
Departments of Pathology, St. Joseph's Health Centre and London Health Science Centre, London Ontario Canada, and Clinical Laboratories Thunder Bay Regional Hospital, Thunder Bay, Ontario.
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Extract

Fibrillary and immunotactoid glomerulonephritis (FGN & IGN) are uncommon glomerular diseases, diagnosed by electron microscopic identification of fibril deposits which are negative by the Congo red stain. Micro-fibrils are similar to normal structures in glomerular basement membrane and matrix and they can be missed by EM. Pathogenesis of these glomerulopathies is unclear but they are not believed to be associated with monoclonal gammopathies as in AL amyloidosis. Immunofluorescense (IF) on FGN indicates the fibrils typically are positive for IgG and both immunoglobulin light chains while IGN fibrils are light chain monoclonal. We did immunogold labeling on four cases of FIGN in to improve the diagnosis of these diseases and investigate fibril protein composition.

Renal biopsies from four patients consisted of one IGN and 3 FGN. Tests included frozen section IF (IgG, IgA, IgM, C3), paraffin section histology (Congo Red) and conventional EM (osmium fixation and epoxy embedding).

Type
Pathology
Information
Microscopy and Microanalysis , Volume 6 , Issue S2: Proceedings: Microscopy & Microanalysis 2000, Microscopy Society of America 58th Annual Meeting, Microbeam Analysis Society 34th Annual Meeting, Microscopical Society of Canada/Societe de Microscopie de Canada 27th Annual Meeting, Philadelphia, Pennsylvania August 13-17, 2000 , August 2000 , pp. 582 - 583
Copyright
Copyright © Microscopy Society of America

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References

References:

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