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Imaging of Vitrified Biological Specimens by Confocal Cryo-Fluorescence Microscopy and Cryo-FIB/SEM Tomography

Published online by Cambridge University Press:  23 September 2015

Alexander Rigort ,
Robert Kirmse ,
Volker Doring ,
Michael Schwertner  and
Ralf Wolleschensky
Alexander Rigort
Affiliation:
Carl Zeiss Microscopy, Jena, Germany,
Robert Kirmse
Affiliation:
Carl Zeiss Microscopy, Jena, Germany,
Volker Doring
Affiliation:
Carl Zeiss Microscopy, Jena, Germany,
Michael Schwertner
Affiliation:
Linkam Scientific Instruments, Tadworth, UK
Ralf Wolleschensky
Affiliation:
Carl Zeiss Microscopy, Jena, Germany,

Abstract

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Type
Abstract
Information
Microscopy and Microanalysis , Volume 21 , Supplement S3: Proceedings of Microscopy & Microanalysis 2015 , August 2015 , pp. 1121 - 1122
Copyright
Copyright © Microscopy Society of America 2015 

References

References:

[1] Schertel, A, et al., Cryo FIB-SEM: volume imaging of cellular ultrastructure in native frozen specimens. J Struct Biol. (2013). Nov; 184 (2): 355–360. doi: 10.1016/j.jsb.2013.09.024.Google Scholar
[2] Kaufmann, R, Hagen, C & Grunewald, K. Fluorescence cryo-microscopy: current challenges and prospects. Current opinion in chemical biology. (2014) 20, 86–91. DOI: 10.1016/j.cbpa.2014.05.007.Google Scholar