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Imaging Bioprinted Model Systems for Metastatic Breast Cancer Studies

Published online by Cambridge University Press:  30 July 2020

Jessica Riesterer ,
Arun Singh ,
Erin Stempinski ,
Steven Adamou ,
Cecilia Bueno ,
Hannah Smith ,
Claudia Lopez  and
Joe Gray
Jessica Riesterer
Affiliation:
Oregon Health and Science University, Portland, Oregon, United States
Arun Singh
Affiliation:
Oregon Health and Science University, Portland, Oregon, United States
Erin Stempinski
Affiliation:
Oregon Health and Science University, Portland, Oregon, United States
Steven Adamou
Affiliation:
Oregon Health and Science University, Portland, Oregon, United States
Cecilia Bueno
Affiliation:
Oregon Health and Science University, Portland, Oregon, United States
Hannah Smith
Affiliation:
Oregon Health and Science University, Portland, Oregon, United States
Claudia Lopez
Affiliation:
Oregon Health and Science University, Portland, Oregon, United States
Joe Gray
Affiliation:
Oregon Health and Science University, Portland, Oregon, United States

Abstract

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Type
3D Scanning Electron Microscopy Imaging of Biological Samples
Information
Microscopy and Microanalysis , Volume 26 , Supplement S2 , August 2020 , pp. 1376 - 1377
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Copyright
Copyright © Microscopy Society of America 2020

References

Johnson, B. et al. Abstract 3296: SMMART: Serial measurements of molecular and architectural responses to therapy. Cancer Research 78, 3296 (2018).Google Scholar
Karnovsky, M.J. A Formaldehyde-Glutaraldehyde Fixative of High Osmolality for Use in Electron Microscopy. Journal of Cell Biology 27, 137-8A (1965).Google Scholar
Hua, Y., Laserstein, P. & Helmstaedter, M. Large-volume en-bloc staining for electron microscopy-based connectomics. Nature Communications 6, 1–7 (2015).10.1038/ncomms8923CrossRefGoogle ScholarPubMed
Riesterer, J.L. et al. A workflow for visualizing human cancer biopsies using large-format electron microscopy. bioRxiv, 675371 (2019).Google Scholar
The authors wish to acknowledge the OHSU Multiscale Microscopy Core for instrument access. Funding was graciously provided by the OHSU Center for Spatial Systems, OHSU University Shared Resources, and the NCI Cancer Systems Biology Measuring, Modeling, and Controlling Heterogeneity (M2CH) Center awarded to Joe Gray (5U54CA2099880).Google Scholar