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Identification of Papilloma-Like Virus Particles in Cell Lines Derived from Green Sea Turtle (Chelonia Mydas) With Fibropapilloma

Published online by Cambridge University Press:  02 July 2020

Yuanan Lu ,
Vivek R. Nerurkar ,
Tina M. Weatherby  and
Richard Yanagihara
Yuanan Lu
Affiliation:
Retrovirology Research Laboratory and Biological Electron Microscope Facility, Pacific Biomedical Research Center, University of Hawaii at Manoa, Honolulu, HI96822
Vivek R. Nerurkar
Affiliation:
Retrovirology Research Laboratory and Biological Electron Microscope Facility, Pacific Biomedical Research Center, University of Hawaii at Manoa, Honolulu, HI96822
Tina M. Weatherby
Affiliation:
Biological Electron Microscope Facility, Pacific Biomedical Research Center, University of Hawaii at Manoa, Honolulu, HI96822
Richard Yanagihara
Affiliation:
Retrovirology Research Laboratory and Biological Electron Microscope Facility, Pacific Biomedical Research Center, University of Hawaii at Manoa, Honolulu, HI96822
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Extract

The near epidemic occurrence of fibropapilloma in green sea turtle (Chelonia my das) (Figure 1) significantly threatens the survival of this species which is protected under the U.S. Endangered Species Act. Although collective evidence suggests a viral etiology, the causative virus of green sea turtle fibropapilloma has not been isolated. To facilitate the isolation and characterization of the causative virus(es), we established 13 cell lines from multiple organs/tissues (tumor, kidney, lung, heart, gall bladder, testis, and skin) of green sea turtles with fibropapilloma. Serial subcultivation of cell lines derived from lungs, testis, eye soft tissues and tumors resulted in the formation of tumor-like aggregates, which attained sizes of 1-2 mm in diameter within two weeks (Figure 2). Media from such cultures, when inoculated onto cells derived from healthy turtle embryos, produced similar tumor-like aggregates, suggesting the presence of a transmissible agent.

Type
Biological Ultrastructure/Microbiology
Information
Microscopy and Microanalysis , Volume 4 , Issue S2: Proceedings: Microscopy & Microanalysis '98, Microscopy Society of America 56th Annual Meeting, Microbeam Analysis Society 32nd Annual Meeting, Atlanta, Georgia July 12-16, 1998 , July 1998 , pp. 1158 - 1159
Copyright
Copyright © Microscopy Society of America

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References

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