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Identification of MHC-I Accessible Proteins from Salmonella Typhimurium

Published online by Cambridge University Press:  02 July 2020

D. Ellefson ,
D. Parker  and
F. Heffron
D. Ellefson*
Affiliation:
Department of Molecular Microbiology and Immunology, Oregon Health Sciences University, Portland, OR97201
D. Parker*
Affiliation:
Department of Molecular Microbiology and Immunology, Oregon Health Sciences University, Portland, OR97201
F. Heffron*
Affiliation:
Department of Molecular Microbiology and Immunology, Oregon Health Sciences University, Portland, OR97201
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*
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Abstract

Intracellular bacterial pathogens such as Salmonella typhimurium secrete proteins into the host cell after infection. These proteins alter the normal structural and metabolic machinery of the host cell and benefit the bacterium by facilitating replication and avoidance of host immune surveillance. Since the host cytoplasmic localization of these proteins infers access to the class-I MHC antigen processing and presentation machinery of the host cell, we collectively refer to these proteins as Class- I Accessible Proteins (CAPs).

The design of vaccines for new and emerging bacterial pathogens is often constrained by the selection of appropriate and specific antigens. While vaccine design is being greatly aided by whole genome analysis of bacterial pathogens, it has been of limited use in the assignment of function and host subcellular localization of a large percentage of bacterial proteins. in addition, analysis of the bacteria/host interaction is further complicated by the complex lifestyle of the pathogen.

Type
Correlative Fluorescent Microscopy and Flow Cytometry Techniques (Organized by R. Smith)
Information
Microscopy and Microanalysis , Volume 7 , Issue S2: Proceedings: Microscopy & Microanalysis 2001, Microscopy Society of America 59th Annual Meeting, Microbeam Analysis Society 35th Annual Meeting, Long Beach, California August 5-9, 2001 , August 2001 , pp. 616 - 617
Copyright
Copyright © Microscopy Society of America 2001

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References

1.Ellefson, D., et al. (2000). Methods Enzymol 326, 516-26.CrossRefGoogle Scholar
2.Porgador, A., et al. (1997) Immunity 6, 715-26.CrossRefGoogle Scholar