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Hydrotalcite Can Prevent the Damaging Effects of Helicobacter Pylori on Gastric Epithelial Cells

Published online by Cambridge University Press:  28 June 2018

Shi Yanyan ,
Guo Yanlei ,
Zhang Ting  and
Ding Shigang Open the ORCID record for Ding Shigang [Opens in a new window]
Shi Yanyan
Affiliation:
Research Center of Clinical Epidemiology, Peking University Third Hospital, Beijing 100191, P.R.China
Guo Yanlei
Affiliation:
Department of Gastroenterology, Peking University Third Hospital, Beijing 100191, P.R.China
Zhang Ting
Affiliation:
Department of Microbiology, Peking University Health Science Center, Beijing 100191, P.R.China
Ding Shigang*
Affiliation:
Department of Gastroenterology, Peking University Third Hospital, Beijing 100191, P.R.China
*
*Author for correspondence: Shigang Ding, E-mail: [email protected]
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Abstract

Background: Helicobacter pylori is a major cause of gastric diseases including gastric cancer. This study was aimed to explore whether hydrotalcite can inhibit H. pylori infection of gastric epithelial cells. Methods: the gastric epithelial cell line GES-1 and the gastric cancer cell line BGC823 were infected with H. pylori at multiplicities of infections (MOIs) of 50:1 and 100:1. Hydrotalcite was added to cell cultures. Cell apoptosis and cell cycle analysis were performed to measure the situation of cell growth. The main changes of cell ultrastructure were observed by transmission electron microscopy. H. pylori cell adhesion was observed by scanning electron microscopy. Results: hydrotalcite could significantly inhibit cell apoptosis of GES-1 and cell proliferation of BGC823 induced by H. pylori infection at an MOI of 50:1. Hydrotalcite treatment protected gastric cells from H. pylori infection, and H. pylori adhesion to gastric cells was reduced. However, hydrotalcite could not reverse damage induced by H. pylori infection at an MOI of 100:1. Conclusion: hydrotalcite can protect gastric cells from H. pylori infection when cell damage is not serious. It can weaken the damage of cells induced by H. pylori and decrease H. pylori adhesion to gastric cells.

Keywords

Helicobacter pylorihydrotalcitegastriccellelectron microscopy
Type
Biological Science Applications
Information
Microscopy and Microanalysis , Volume 24 , Issue 3 , June 2018 , pp. 277 - 283
Copyright
© Microscopy Society of America 2018 

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Footnotes

Cite this article: Yanyan Shi, Yanlei Guo, Ting Zhang and Shigang Ding (2018) Hydrotalcite Can Prevent the Damaging Effects of Helicobacter Pylori on Gastric Epithelial Cells. Microsc Microanal24(3): 277–283. doi: 10.1017/S1431927618000314

References

Abudayyak, M, Oztas, E, Arici, M and Ozhan, G (2017) Investigation of the toxicity of bismuth oxide nanoparticles in various cell lines. Chemosphere 169, 117–123.CrossRefGoogle ScholarPubMed
Ajani, JA, Lee, J, Sano, T, Janjigian, YY, Fan, D and Song, S (2017) Gastric adenocarcinoma. Nat Rev Dis Primers 3, 17036.Google Scholar
Bussiere, FI, Chaturvedi, R, Asim, M, Hoek, KL, Cheng, Y, Gainor, J, Scholz, A, Khan, WN and Wilson, KT (2006) Low multiplicity of infection of Helicobacter pylori suppresses apoptosis of B lymphocytes. Cancer Res 66(13), 6834–6842.Google Scholar
Correa, P (1995) Helicobacter pylori and gastric carcinogenesis. Am J Surg Pathol 19(Suppl 1), S37–43.Google Scholar
Correa, P and Piazuelo, MB (2008) Natural history of Helicobacter pylori infection. Dig Liver Dis 40(7), 490–496.Google Scholar
Correa, P and Piazuelo, MB (2012) The gastric precancerous cascade. J Dig Dis 13(1), 2–9.CrossRefGoogle ScholarPubMed
David, S and Meltzer, SJ (2010) Stomach – genetic and epigenetic alterations of preneoplastic and neoplastic lesions. Cancer Biomark 9(1-6), 493–507.Google Scholar
Doulberis, M, Kotronis, G, Thomann, R, Polyzos, SA, Boziki, M, Gialamprinou, D, Deretzi, G, Katsinelos, P and Kountouras, J (2018) Impact of Helicobacter pylori on Alzheimer’s disease: What do we know so far? Helicobacter 23, e12454.CrossRefGoogle ScholarPubMed
Ford, AC, Forman, D, Hunt, RH, Yuan, Y and Moayyedi, P (2014) Helicobacter pylori eradication therapy to prevent gastric cancer in healthy asymptomatic infected individuals: Systematic review and meta-analysis of randomised controlled trials. BMJ 348, g3174.CrossRefGoogle ScholarPubMed
Gooz, M, Shaker, M, Gooz, P and Smolka, AJ (2003) Interleukin 1beta induces gastric epithelial cell matrix metalloproteinase secretion and activation during Helicobacter pylori infection. Gut 52(9), 1250–1256.CrossRefGoogle ScholarPubMed
Huang, HK, Wang, JH, Lei, WY, Chen, CL, Chang, CY and Liou, LS (2018) Helicobacter pylori infection is associated with an increased risk of Parkinson’s disease: A population-based retrospective cohort study. Parkinsonism Relat Disord 47, 26–31.Google Scholar
Kangwan, N, Park, JM, Kim, EH and Hahm, KB (2014) Quality of healing of gastric ulcers: Natural products beyond acid suppression. World J Gastrointest Pathophysiol 5(1), 40–47.Google Scholar
Lee, YC, Chiang, TH, Chou, CK, Tu, YK, Liao, WC, Wu, MS and Graham, DY (2016) Association between Helicobacter pylori eradication and gastric cancer incidence: A systematic review and meta-analysis. Gastroenterology 150(5), 1113–1124 e1115.Google Scholar
Leussink, BT, Slikkerveer, A, Engelbrecht, MR, van der Voet, GB, Nouwen, EJ, de Heer, E, de Broe, ME, de Wolff, FA and Bruijn, JA (2001) Bismuth overdosing-induced reversible nephropathy in rats. Arch Toxicol 74(12), 745–754.Google Scholar
Malfertheiner, P, Megraud, F, O’Morain, CA, Gisbert, JP, Kuipers, EJ, Axon, AT, Bazzoli, F, Gasbarrini, A, Atherton, J, Graham, DY, Hunt, R, Moayyedi, P, Rokkas, T, Rugge, M, Selgrad, M, Suerbaum, S, Sugano, K and El-Omar, EM (2017) Management of Helicobacter pylori infection-the Maastricht V/Florence Consensus Report. Gut 66(1), 6–30.Google Scholar
McColl, KE (2010) Clinical practice. Helicobacter pylori infection. N Engl J Med 362(17), 1597–1604.Google Scholar
Megraud, F, Coenen, S, Versporten, A, Kist, M, Lopez-Brea, M, Hirschl, AM, Andersen, LP, Goossens, H and Glupczynski, Y (2013) Helicobacter pylori resistance to antibiotics in Europe and its relationship to antibiotic consumption. Gut 62(1), 34–42.Google Scholar
Mimuro, H, Suzuki, T, Nagai, S, Rieder, G, Suzuki, M, Nagai, T, Fujita, Y, Nagamatsu, K, Ishijima, N, Koyasu, S, Haas, R and Sasakawa, C (2007) Helicobacter pylori dampens gut epithelial self-renewal by inhibiting apoptosis, a bacterial strategy to enhance colonization of the stomach. Cell Host Microbe 2(4), 250–263.CrossRefGoogle ScholarPubMed
Ng, QX, Venkatanarayanan, N, De Deyn, M, Ho, CYX, Mo, Y and Yeo, WS (2018) A meta-analysis of the association between Helicobacter pylori (H. pylori) infection and hyperemesis gravidarum. Helicobacter 23, e12455.Google Scholar
Shi, Y, Liu, L, Zhang, T, Shen, L, Zhang, J, Zhang, Y, Wang, X, Yang, S, Lu, F, Chen, X and Ding, S (2013) The involvement of Helicobacter pylori thioredoxin-1 in gastric carcinogenesis. J Med Microbiol 62(Pt 8), 1226–1234.CrossRefGoogle ScholarPubMed
Sjomina, O, Heluwaert, F, Moussata, D and Leja, M (2017) Helicobacter pylori infection and nonmalignant diseases. Helicobacter 22(Suppl 1), e12408.Google Scholar
Sugano, K, Tack, J, Kuipers, EJ, Graham, DY, El-Omar, EM, Miura, S, Haruma, K, Asaka, M, Uemura, N and Malfertheiner, P (2015) Kyoto global consensus report on Helicobacter pylori gastritis. Gut 64(9), 1353–1367.Google Scholar
Tarnawski, A, Ahluwalia, A and Jones, MK (2013) Gastric cytoprotection beyond prostaglandins: Cellular and molecular mechanisms of gastroprotective and ulcer healing actions of antacids. Curr Pharm Des 19(1), 126–132.Google Scholar
Tarnawski, A, Pai, R, Itani, R and Wyle, FA (1999) The antacid Talcid adsorbs and neutralizes all proteins secreted by H. pylori including VacA cytotoxin: A new mechanism for its ulcer-healing action? Digestion 60(5), 449–455.Google Scholar
Thung, I, Aramin, H, Vavinskaya, V, Gupta, S, Park, JY, Crowe, SE and Valasek, MA (2016) Review article: The global emergence of Helicobacter pylori antibiotic resistance. Aliment Pharmacol Ther 43(4), 514–533.Google Scholar
Valenzuela, MA, Canales, J, Corvalan, AH and Quest, AF (2015) Helicobacter pylori-induced inflammation and epigenetic changes during gastric carcinogenesis. World J Gastroenterol 21(45), 12742–12756.CrossRefGoogle ScholarPubMed
Wroblewski, LE and Peek, RM Jr. (2007) Orchestration of dysregulated epithelial turnover by a manipulative pathogen. Cell Host Microbe 2(4), 209–211.CrossRefGoogle ScholarPubMed
Xiao, YL, Nie, YQ, Hou, XH, Xie, PY, Fang, JY, Yuan, YZ, Zhou, LY, Zhao, NQ and Chen, MH (2013) The efficacy, safety and cost-effectiveness of hydrotalcite versus esomeprazole in on-demand therapy of NERD: A multicenter, randomized, open-label study in China. J Dig Dis 14(9), 463–468.CrossRefGoogle Scholar
Yang, RQ, Mao, H, Huang, LY, Su, PZ and Lu, M (2017) Effects of hydrotalcite combined with esomeprazole on gastric ulcer healing quality: A clinical observation study. World J Gastroenterol 23(7), 1268–1277.Google Scholar
Yu, BP, Sun, J, Li, MQ, Luo, HS and Yu, JP (2003) Preventive effect of hydrotalcite on gastric mucosal injury in rats induced by taurocholate. World J Gastroenterol 9(7), 1427–1430.Google Scholar