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High-resolution Imaging of Reconstituted Protein-DNA Complexes Using Phase Plate Electron Cryo Microscopy

Published online by Cambridge University Press:  25 July 2016

Sara Sandin ,
Radostin Danev ,
Juergen M. Plitzko ,
Eugene Chua Yue Dao ,
Vinod Kumar Vogirala  and
Andrew See Weng Wong
Sara Sandin
Affiliation:
School of Biological Sciences, Nanyang Technological University, Singapore NTU Institute of Structural Biology, Nanyang Technological University, Singapore
Radostin Danev
Affiliation:
Department of Molecular Structural Biology, Max Planck Institute of Biochemistry, Martinsried, Germany
Juergen M. Plitzko
Affiliation:
Department of Molecular Structural Biology, Max Planck Institute of Biochemistry, Martinsried, Germany
Eugene Chua Yue Dao
Affiliation:
School of Biological Sciences, Nanyang Technological University, Singapore NTU Institute of Structural Biology, Nanyang Technological University, Singapore
Vinod Kumar Vogirala
Affiliation:
School of Biological Sciences, Nanyang Technological University, Singapore NTU Institute of Structural Biology, Nanyang Technological University, Singapore
Andrew See Weng Wong
Affiliation:
School of Biological Sciences, Nanyang Technological University, Singapore NTU Institute of Structural Biology, Nanyang Technological University, Singapore

Abstract

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Type
Abstract
Information
Microscopy and Microanalysis , Volume 22 , Supplement S3: Proceedings of Microscopy & Microanalysis 2016 , July 2016 , pp. 68 - 69
Copyright
© Microscopy Society of America 2016 

References

References:

[1] Luger, K, Dechassa, ML & Tremethick, DJ Nat Rev Mol Cell Biol. 13(7 (2012). p. 436–447.Google Scholar
[2] Danev, R, et al, Proc Natl Acad Sci U S A. 111(44 (2014). p. 15635–15640.Google Scholar
[3] Khoshouei, M, et al, Nat Commun. 7 (2016). p. 10534.Google Scholar
[4] S Sandin, ECY Dao, VK Vogirala and ASW Wong acknowledge funding from Nanyang Technological University and Singapore Ministry of Education Academic Research Fund Tier 3 [MOE2012-T3-1-001], lead-PI D Rhodes. We thank L Nordenskiold’s group for their kind contribution of histones used in this study. We thank M Khoshouei, W Baumeister, C Davey and SHW Scheres for help and advice.Google Scholar