Hostname: page-component-78c5997874-dh8gc Total loading time: 0 Render date: 2024-11-02T20:12:10.405Z Has data issue: false hasContentIssue false
  • English
  • Français

High-Resolution Ex Vivo Tissue Clearing, Lightsheet Imaging, and Data Analysis to Support Macromolecular Drug and Biomarker Distribution in Whole Organs and Tumors

Published online by Cambridge University Press:  22 July 2022

Niyanta Kumar ,
Petr Hrobař ,
Martin Vagenknecht ,
Jindrich Soukup ,
Peter Bloomingdale ,
Tomoko Freshwater ,
Sophia Bardehle ,
Roman Peter ,
Nadia Patterson  and
Ruban Mangadu
...Show all authors
Niyanta Kumar*
Affiliation:
ADME & Discovery Toxicology, Merck & Co. Inc., West Point, PA, United States
Petr Hrobař
Affiliation:
Data Science & Scientific Informatics, MSD, Prague, Czech Republic
Martin Vagenknecht
Affiliation:
Data Science & Scientific Informatics, MSD, Prague, Czech Republic
Jindrich Soukup
Affiliation:
Data Science & Scientific Informatics, MSD, Prague, Czech Republic
Peter Bloomingdale
Affiliation:
Quantitative Pharmacology and Pharmacometrics, Merck & Co. Inc., Boston, MA, United States
Tomoko Freshwater
Affiliation:
Quantitative Pharmacology and Pharmacometrics, Merck & Co. Inc., Rahway, NJ, United States
Sophia Bardehle
Affiliation:
Neuroscience, Merck & Co. Inc., Boston, MA, United States
Roman Peter
Affiliation:
Data Science & Scientific Informatics, MSD, Prague, Czech Republic
Nadia Patterson
Affiliation:
ADME & Discovery Toxicology, Merck & Co. Inc., West Point, PA, United States
Ruban Mangadu
Affiliation:
Immuno-Oncology, Merck & Co. Inc., San Francisco, CA, United States
Cinthia Pastuskovas
Affiliation:
ADME & Discovery Toxicology, Merck & Co. Inc., San Francisco, CA, United States
Mark Cancilla
Affiliation:
ADME & Discovery Toxicology, Merck & Co. Inc., West Point, PA, United States
*
*Corresponding author: [email protected]

Abstract

An abstract is not available for this content so a preview has been provided. As you have access to this content, a full PDF is available via the ‘Save PDF’ action button.
Image of the first page of this content. For PDF version, please use the ‘Save PDF’ preceeding this image.'
Type
Imaging, Microscopy, and Micro/Nano-Analysis of Pharmaceutical, Biopharmaceutical, and Medical Health Products - Research, Development, Analysis, Regulation, and Commercialization
Information
Microscopy and Microanalysis , Volume 28 , Supplement S1 , August 2022 , pp. 1436 - 1437
Check for updates
Copyright
Copyright © Microscopy Society of America 2022

References

Chung, K., et al. Structural and molecular interrogation of intact biological systems. Nature 497, 332-337, doi:10.1038/nature12107 (2013).CrossRefGoogle ScholarPubMed
Liebmann, T., et al. Three-dimensional study of Alzheimer's disease hallmarks using the iDISCO clearing method. Cell reports 16, 1138-1152 (2016).CrossRefGoogle ScholarPubMed
Zhao, S., et al. Cellular and molecular probing of intact human organs. Cell 180, 796-812. e719 (2020).CrossRefGoogle ScholarPubMed
Dobosz, M., Ntziachristos, V., Scheuer, W. & Strobel, S. Multispectral fluorescence ultramicroscopy: three-dimensional visualization and automatic quantification of tumor morphology, drug penetration, and antiangiogenic treatment response. Neoplasia 16, 1-13, doi:10.1593/neo.131848 (2014).CrossRefGoogle ScholarPubMed
Belthangady, C. & Royer, L. A. Applications, promises, and pitfalls of deep learning for fluorescence image reconstruction. Nature methods 16, 1215-1225 (2019).CrossRefGoogle ScholarPubMed