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High-precision Cryogenic Correlative Light and Electron Microscopy Using Two Independent Alignment Features and an Easy-to-use Toolkit to Study Virus-infected Mammalian Cells

Published online by Cambridge University Press:  30 July 2020

Jae Yang ,
Bryan Sibert ,
Matthew Larson ,
Samantha Shrum  and
Elizabeth Wright
Jae Yang
Affiliation:
University of Wisconsin-Madison, Madison, Wisconsin, United States
Bryan Sibert
Affiliation:
University of Wisconsin-Madison, Madison, Wisconsin, United States
Matthew Larson
Affiliation:
University of Wisconsin-Madison, Madison, Wisconsin, United States
Samantha Shrum
Affiliation:
University of Wisconsin-Madison, Madison, Wisconsin, United States
Elizabeth Wright
Affiliation:
University of Wisconsin-Madison, Madison, Wisconsin, United States

Abstract

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Type
Correlative and Multimodal Microscopy and Imaging of Physical, Environmental, and Biological Sciences
Information
Microscopy and Microanalysis , Volume 26 , Supplement S2 , August 2020 , pp. 1598 - 1601
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Copyright
Copyright © Microscopy Society of America 2020

References

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This research was supported in part by funds from the University of Wisconsin, Madison and the National Institutes of Health (R01GM114561 and R01GM104540-03S1) to E.R.W. The authors gratefully acknowledge use of facilities and instrumentation at the UW-Madison Wisconsin Centers for Nanoscale Technology (wcnt.wisc.edu) partially supported by the NSF through the University of Wisconsin Materials Research Science and Engineering Center (DMR-1720415). A portion of this research was supported by NIH grant U24GM129547 and performed at the PNCC at OHSU and accessed through EMSL (grid.436923.9), a DOE Office of Science User Facility sponsored by the Office of Biological and Environmental Research.Google Scholar