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High Spatial and High Spectral Resolution FTIR Spectroscopic Imaging of Biological Materials

Published online by Cambridge University Press:  02 July 2020

E.N. Lewis ,
L.H. Kidder  and
I.W. Levin
E.N. Lewis
Affiliation:
Laboratory of Chemical Physics, National Institutes of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD20892-0510
L.H. Kidder
Affiliation:
Laboratory of Chemical Physics, National Institutes of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD20892-0510
I.W. Levin
Affiliation:
Laboratory of Chemical Physics, National Institutes of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD20892-0510
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Extract

Infrared spectroscopy has been used to probe a variety of biological systems including for example, the determination of diseased states and the investigation of foreign inclusions in biologicals. The technique generates qualitative and quantitative information on the structure and dynamics of samples, including lipids, proteins, and non-biological constituents. The coupling of imaging modalities with spectroscopic techniques adds a new dimension to sample analysis in both the spectroscopic and spatial domains. Using a spectroscopic imaging system that incorporates a step-scan interferometer, microscope, and infrared sensitive arrays, we have investigated a variety of biological samples. This seamless combination of spectroscopy for molecular analysis with the power of visualization generates chemically specific images while simultaneously obtaining high resolution spectra for each detector pixel. The spatial resolution of the images approaches the diffraction limit for mid-infrared wavelengths, while the spectral resolution is determined by the interferometer and can be 4 cm−1 or higher.

Type
Optical Microanalysis
Information
Microscopy and Microanalysis , Volume 3 , Issue S2: Proceedings: Microscopy & Microanalysis '97, Microscopy Society of America 55th Annual Meeting, Microbeam Analysis Society 31st Annual Meeting, Histochemical Society 48th Annual Meeting, Cleveland, Ohio, August 10-14, 1997 , August 1997 , pp. 831 - 832
Copyright
Copyright © Microscopy Society of America 1997

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