Hostname: page-component-586b7cd67f-g8jcs Total loading time: 0 Render date: 2024-11-25T00:29:58.880Z Has data issue: false hasContentIssue false
  • English
  • Français

A Freeze-Fracture Study of Nuclear Pore Complex Structure in Intact Dunaliella Cells

Published online by Cambridge University Press:  02 July 2020

M.V. Parthasarathy  and
C.S. Daugherty
M.V. Parthasarathy
Affiliation:
Department of Plant Biology, Cornell University, Ithaca, NY14853
C.S. Daugherty
Affiliation:
Department of Plant Biology, Cornell University, Ithaca, NY14853
Get access

Extract

The nuclear envelope in all eukaryotic cells is perforated by nuclear pores, that consist of the nuclear pore complex (NPC). The NPC is a highly complex supramolecular structure that plays a pivotal role in the trafficking of macromolecules and particles between the nucleus and the cytoplasm. During the past two decades a large body of information has accumulated on both the structure and role of the NPC. The diameter of NPC is about 120 nm and is composed of approximately 100 proteins.

The NPC structure has been elucidated using various electron microscopy techniques. The basic structure of the NPC is comprised of three concentric rings, each displaying an 8-fold symmetry. The cytoplasmic and nuclear rings are vertically integrated by eight spokes that approximately span the width of the nuclear envelope. A set of eight fibers extends from the nucleoplasmic ring into the nucleus, and are joined at the distal ends by a ring to form the so called basket.

Type
Biological Structure (Cells, Tissues, Organ Systems)
Information
Microscopy and Microanalysis , Volume 6 , Issue S2: Proceedings: Microscopy & Microanalysis 2000, Microscopy Society of America 58th Annual Meeting, Microbeam Analysis Society 34th Annual Meeting, Microscopical Society of Canada/Societe de Microscopie de Canada 27th Annual Meeting, Philadelphia, Pennsylvania August 13-17, 2000 , August 2000 , pp. 882 - 883
Copyright
Copyright © Microscopy Society of America

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

References:

1.Ris, H.. EMSA Bull. 21 (1991) 54.Google Scholar
2.Jarnik, M.. and Aebi, U.. J. Struct. Biol. 107 (1991) 291.CrossRefGoogle Scholar
3.Goldberg, M.W., and Allen, T.D.. J. Cell Sci. 106 (1993) 261.Google Scholar
4.Goldberg, M.W. et al. J. Cell Sci. 110 (1997) 409.Google Scholar
5.Goldberg, M.W. et al. Protoplasma 209 (1999) 144.CrossRefGoogle Scholar
6. This work was conducted at the Cornell Integrated Microscopy Center.Google Scholar