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Freeze Drying Method with Gaseous Nitrogen to Preserve Fine Ultrastructure of Biological Organizations for Scanning Electron Microscopy, Helium Ion Beam Microscopy and Fluorescence Microscopy

Published online by Cambridge University Press:  25 July 2016

Kunihiro Uryu ,
Nadine Soplop ,
Devrim Acehan ,
Benjamin Y. Winer ,
Vincent A. Fischetti ,
Timothy Sheahan ,
Charles M. Rice ,
Mayla Hsu ,
Melissa Robbiani  and
Gaetano Santulli
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Kunihiro Uryu
Affiliation:
Electron Microscopy Resource Center
Nadine Soplop
Affiliation:
Electron Microscopy Resource Center
Devrim Acehan
Affiliation:
Electron Microscopy Resource Center
Benjamin Y. Winer
Affiliation:
Laboratory of Bacterial Pathogenesis and Immunology
Vincent A. Fischetti
Affiliation:
Laboratory of Bacterial Pathogenesis and Immunology
Timothy Sheahan
Affiliation:
Laboratory of Virology and Infectious Disease, The Rockefeller University, New York, New York, USA
Charles M. Rice
Affiliation:
Laboratory of Virology and Infectious Disease, The Rockefeller University, New York, New York, USA
Mayla Hsu
Affiliation:
Center for Biomedical Research, Population Council, New York, New York, USA
Melissa Robbiani
Affiliation:
Center for Biomedical Research, Population Council, New York, New York, USA
Gaetano Santulli
Affiliation:
Department of Physiology and Cellular Biophysics, The Clyde and Helen Wu Center for Molecular Cardiology, Columbia University Medical Center, New York, New York, USA.
Hana Totary-Jain
Affiliation:
Department of Physiology and Cellular Biophysics, The Clyde and Helen Wu Center for Molecular Cardiology, Columbia University Medical Center, New York, New York, USA. Department of Molecular Pharmacology & Physiology, University of South Florida Morsani College of Medicine, Tampa, Florida, USA.
Bernhard Goetze
Affiliation:
Ion Microscopy Innovation Center, Zeiss Microscopy LLC, Peabody, Massachusetts, USA.
Maria-Teresa Catanese
Affiliation:
Laboratory of Virology and Infectious Disease, The Rockefeller University, New York, New York, USA Department of Infectious Diseases, King's College London School of Medicine, Guy's Hospital, Great Maze Pond, LondonSE1 9RT, UK

Abstract

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Abstract
Information
Microscopy and Microanalysis , Volume 22 , Supplement S3: Proceedings of Microscopy & Microanalysis 2016 , July 2016 , pp. 1142 - 1143
Copyright
© Microscopy Society of America 2016 

References

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[7] Acknowledgements: The current study was supported by The Rockefeller University, the American Heart Association Scientist Developmental Grant 15SDG25300007 (Santulli), the United States Agency for International Development Cooperative Agreement GPO-A-00-04-00019-00 (Robbiani), the NIH grant AI098645 (Robbiani), the TNPRC NIH base grant P51 OD011104-52 (Robbiani), NIH R01 AI072613 (Rice), NIH R01 CA057973 (Rice), NHLBI/NIH R00HL109133 (Totary-Jain) and ContraFect Corporation (Fischetti).Google Scholar