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Fluorescent and Electron Microscopic Studies Suggest That Confronting Cisternae Arise from Nuclear Envelope in Mitotic Cells

Published online by Cambridge University Press:  02 July 2020

J. R. Palisano ,
J. L. Thacker ,
C. S. Piromalli ,
A. M. Morrison  and
J. E. Tate
J. R. Palisano
Affiliation:
Department of Biology, The University of the South, Sewanee, TN37383-1000
J. L. Thacker
Affiliation:
University of Tennessee School of Medicine, Memphis, TN38105
C. S. Piromalli
Affiliation:
University of Tennessee School of Medicine, Memphis, TN38105 Watson Fellow, India
A. M. Morrison
Affiliation:
Department of Biology, The University of the South, Sewanee, TN37383-1000
J. E. Tate
Affiliation:
Department of Biology, The University of the South, Sewanee, TN37383-1000
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Extract

The origin and role of confronting cisternae (CC) have been clouded in mystery since their discovery in 1955. Early investigations suggested that CC arose from either nuclear envelope (NE) or by stacking of endoplasmic reticulum (ER) cisternae. Electron microscopic studies of mitotic HeLa cells suggest that CC are fragments of NE (Fig. 1) that arise as portions of the NE fold back on one another. Because lamin B is a NE specific protein, a monoclonal antibody to lamin B was used to probe the origin of CC. The monoclonal antibody to lamin B was then detected by utilizing a secondary antibody conjugated to the fluorochrome rhodamine which fluoresces red when exposed to UV light. Fluorescent microscopy of interphase HeLa cells demonstrates that only the NE fluoresces red when the cells are probed with a monoclonal antibody to lamin B (Fig. 2). There is no label localized in the cytoplasm which supports the evidence that lamin B is only localized to the NE in interphase cells.

Type
Dynamics of Cellular Membrane Traffic
Information
Microscopy and Microanalysis , Volume 4 , Issue S2: Proceedings: Microscopy & Microanalysis '98, Microscopy Society of America 56th Annual Meeting, Microbeam Analysis Society 32nd Annual Meeting, Atlanta, Georgia July 12-16, 1998 , July 1998 , pp. 1028 - 1029
Copyright
Copyright © Microscopy Society of America

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