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First Report of a Putative Cyanophage, Mc-1, of Microcoleus Sp.

Published online by Cambridge University Press:  02 July 2020

J. R. Rosowski ,
J. J. Shaffer ,
E. L. Martin ,
T. A. Kokjohn  and
K. W. Lee
J. R. Rosowski
Affiliation:
School of Biological Sciences, University of Nebraska-Lincoln, Lincoln, NE68588-0118.
J. J. Shaffer
Affiliation:
School of Biological Sciences, University of Nebraska-Lincoln, Lincoln, NE68588-0118.
E. L. Martin
Affiliation:
School of Biological Sciences, University of Nebraska-Lincoln, Lincoln, NE68588-0118.
T. A. Kokjohn
Affiliation:
Midwestern University, 19555 North 59th Avenue, Glendale, Arizona85308. U.S.A.
K. W. Lee
Affiliation:
School of Biological Sciences, University of Nebraska-Lincoln, Lincoln, NE68588-0118.
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Extract

A current review (1) lists 21 cyanophages of coccoid and filamentous heterocystic and non-heterocystic blue-green algae (BGA). The capsid diameters of these viruses range from 50-90 nm, and none have been previously reported for Microcoleus,a sheath-producing, filamentous, surface colonizing BGA (Fig. 1), of the order Oscillatoriales, and class Cyanophyceae (= Cyanobacteria). From a freshwater culture of Microcoleussp. we report here a putative cyanophage with a side-to-side hexagonal capsid diameter of 140 nm, based on measurements from thin sections of algal filaments prepared for transmission electron microscopy (TEM).

An earlier report (2) documented the ultrastructure of mucilage secretion, and its role in sheath, trichome and filament formation in an isolate of the BGA Microcoleus sp. grown in soil-water medium. Now we report large cyanophage-like particles in a majority of cells making up the trichomes. These putative hexagonal viral capsids (Figs. 2-5) are mainly clustered in the center or to one side of the nucleoplasm.

Type
Microbiology
Information
Microscopy and Microanalysis , Volume 5 , Issue S2: Proceedings: Microscopy & Microanalysis '99, Microscopy Society of America 57th Annual Meeting, Microbeam Analysis Society 33rd Annual Meeting, Portland, Oregon August 1-5, 1999 , August 1999 , pp. 1142 - 1143
Copyright
Copyright © Microscopy Society of America

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References

1.Martin, E. L. and Kokjohn, T.A., Encyclopedia of Virology, 2nd Ed., Academic Press (1999), in press.Google Scholar
2.Rosowski, J. R. et al., Nova Hedwigia, Beiheft 112(1996)105.Google Scholar
3.Van Etten, J. L. et al., Virology 140(1985)135.CrossRefGoogle Scholar
4.Miller, R.V. et al., Virology 59(1974)566.CrossRefGoogle Scholar