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Exploring the Contribution of Mycobacteria Characteristics in Their Interaction with Human Macrophages

Published online by Cambridge University Press:  24 June 2013

Carla Silva ,
Joao Perdigao ,
Elsa Alverca ,
António P. Alves de Matos ,
Patricia A. Carvalho ,
Isabel Portugal  and
Luisa Jordao
Carla Silva
Affiliation:
Centro de Patogénese Molecular, URIA, Faculdade de Farmácia da Universidade de Lisboa, Av. Prof. Gama Pinto, 1649-003 Lisboa, Portugal
Joao Perdigao
Affiliation:
Centro de Patogénese Molecular, URIA, Faculdade de Farmácia da Universidade de Lisboa, Av. Prof. Gama Pinto, 1649-003 Lisboa, Portugal
Elsa Alverca
Affiliation:
Instituto Nacional de Saúde Dr Ricardo Jorge (INSA), Departamento de Saúde Ambiental, Av. Padre Cruz, 1649-016 Lisboa, Portugal
António P. Alves de Matos
Affiliation:
Serviço de Anatomia Patológica, Centro Hospitalar de Lisboa Central, Hospital Curry Cabral, R. da Beneficência 8, 1069-166 Lisboa, Portugal
Patricia A. Carvalho
Affiliation:
ICEMS, Departamento de Bioengenharia, Instituto Superior Técnico, Universidade Técnica de Lisboa, Av. Rovisco Pais, 1049-001 Lisboa, Portugal
Isabel Portugal
Affiliation:
Centro de Patogénese Molecular, URIA, Faculdade de Farmácia da Universidade de Lisboa, Av. Prof. Gama Pinto, 1649-003 Lisboa, Portugal
Luisa Jordao*
Affiliation:
INSA, Departamento de Doenças Infeciosas, Av. Padre Cruz, 1649-016 Lisboa, Portugal
*
*Corresponding author. E-mail: [email protected]
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Abstract

Tuberculosis (TB) is a major health problem. The emergence of multidrug resistant (MDR) Mycobacterium tuberculosis (Mtb) isolates confounds treatment strategies. In Portugal, cases of MDR-TB are reported annually with an increased incidence noted in Lisbon. The majority of these MDR-TB cases are due to closely related mycobacteria known collectively as the Lisboa family and Q1 cluster. Genetic determinants linked to drug resistance have been exhaustively studied resulting in the identification of family and cluster specific mutations. Nevertheless, little is known about other factors involved in development of mycobacteria drug resistance. Here, we complement genetic analysis with the study of morphological and structural features of the Lisboa family and Q1 cluster isolates by using scanning and transmission electron microscopy. This analysis allowed the identification of structural differences, such as cell envelope thickness, between Mtb clinical isolates that are correlated with antibiotic resistance. The infection of human monocyte derived macrophages allowed us to document the relative selective advantage of the Lisboa family isolates over other circulating Mtb isolates.

Keywords

Mycobacteriumtuberculosishost–pathogen interactiondrug resistanceTEMSEMMIRUScell envelope
Type
Portuguese Society for Microscopy
Information
Microscopy and Microanalysis , Volume 19 , Issue 5 , October 2013 , pp. 1159 - 1169
Copyright
Copyright © Microscopy Society of America 2013 

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