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Examination of Frozen, Hydrated Mites Using Low Temperature Field Emission Scanning Electron Microscopy

Published online by Cambridge University Press:  02 July 2020

Ronald Ochoa ,
Eric F. Erbe ,
Jeffery S. Pettis  and
William P. Wergin
Ronald Ochoa
Affiliation:
Systematic Entomology Laboratory, USDA ARS, BARC-West, Beltsville Agricultural Research Center, Beltsville, MD20705
Eric F. Erbe
Affiliation:
hematology Laboratory, USDA ARS, BARC-West, Beltsville Agricultural Research Center, Beltsville, MD20705
Jeffery S. Pettis
Affiliation:
Bee Research Laboratory, USDA ARS, BARC-East, Beltsville Agricultural Research Center, Beltsville, MD20705.
William P. Wergin
Affiliation:
hematology Laboratory, USDA ARS, BARC-West, Beltsville Agricultural Research Center, Beltsville, MD20705
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Extract

Mites, the second largest arthropod group after insects, occupy every conceivable terrestrial and aquatic habitat in our environment. They feed on plants, infest food products such as meat, cheese and grains, parasitize invertebrates and vertebrates, and transmit fungal, bacterial, rickettsial and viral diseases. Estimates indicate that as many as 1,000,000 species of mites may exist; however, partly because of their microscopic size, only about 40,000 species have been described and classified. During the last 30 years, researchers have increasingly utilized the greater magnification and depth of field available in a conventional scanning electron microscope (SEM) to supplement descriptions of mites that were historically based on light microscopic observations. In addition, this technique provided a better understanding of the relative positions and functionality of organs and improved attempts to elucidate their biology. However, before mites can be imaged with a conventional SEM, they are typically chemically fixed, dehydrated and/or thoroughly dried.

Type
Biological Structure (Cells, Tissues, Organ Systems)
Information
Microscopy and Microanalysis , Volume 6 , Issue S2: Proceedings: Microscopy & Microanalysis 2000, Microscopy Society of America 58th Annual Meeting, Microbeam Analysis Society 34th Annual Meeting, Microscopical Society of Canada/Societe de Microscopie de Canada 27th Annual Meeting, Philadelphia, Pennsylvania August 13-17, 2000 , August 2000 , pp. 874 - 875
Copyright
Copyright © Microscopy Society of America

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References

References:

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