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Epidermal Keratinocyte Differentiation is Disrupted in Mice Lacking The Full Length Extracellular Calcium-Sensing Receptor

Published online by Cambridge University Press:  02 July 2020

László G. Kömüves ,
Jonathan D. Harris ,
Chrystal Ho  and
Daniel D. Bikle
László G. Kömüves
Affiliation:
University of California San Francisco, VA Medical Center,
Jonathan D. Harris
Affiliation:
University of CaliforniaSan Francisco, CA94121
Chrystal Ho
Affiliation:
University of California San Francisco, Harvard Medical School, Boston, MA02115
Daniel D. Bikle
Affiliation:
University of CaliforniaSan Francisco, CA94121
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Extract

The importance of the extracellular calcium-sensing receptor (CaR) in the stringent control of extracellular Ca2+ concentration is well established. However, the presence of CaR in tissues not directly involved in regulating mineral ion homeostasis suggests a role for CaR in local regulation of cellular functions. Although extracellular Ca2+ regulates the differentiation of keratinocytes, the role of CaR in the epidermis is not established. In this work using knockout mice lacking full length CaR, we sought to determine the role of CaR in epidermal differentiation.

Dorsal skin of Casr−/− knockout mice lacking full length CaR, and Casr+/+ (wild type) control mice, aged 4 to 7 days after birth was fixed in 4% formaldehyde in PBS, and in 2.5% glutaraldehyde and 2% formaldehyde in 0.1 M cacodylate buffer. The samples were embedded in paraffin (for immunohistochemistry and for in situ hybridization) or in Spurr’s or LR White resins. Digoxigenin labeled antisense and sense RNA probes for loricrin and filaggrin were used for in situ hybridization.

Type
Developmental and Reproductive Biology
Information
Microscopy and Microanalysis , Volume 3 , Issue S2: Proceedings: Microscopy & Microanalysis '97, Microscopy Society of America 55th Annual Meeting, Microbeam Analysis Society 31st Annual Meeting, Histochemical Society 48th Annual Meeting, Cleveland, Ohio, August 10-14, 1997 , August 1997 , pp. 185 - 186
Copyright
Copyright © Microscopy Society of America 1997

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References

1.Brown, E.M.et al., England, N.J. Med. 333 (1995) 234.Google Scholar
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3. The cDNAs for loricrin and filaggrin, and antibody for loricrin were provided by Dr.Yuspa, S.(NIH).Google Scholar