No CrossRef data available.
We use cookies to distinguish you from other users and to provide you with a better experience on our websites. Close this message to accept cookies or find out how to manage your cookie settings.
We use cookies to distinguish you from other users and to provide you with a better experience on our websites. Close this message to accept cookies or find out how to manage your cookie settings.
Published online by Cambridge University Press: 02 July 2020
Deregulation of epidermal growth factor receptor (EGFR) gene expression is belived to be a major factor in the development of epithelial cell tumorigenesis, including hepatic carcinomas. To examine EGFR expression within the course of liver cirrhosis, we have identified its hepatic mRNA distribution using a biotinylated antisense oligonucleotide probe.
In this study liver samples from liver transplantation recipients were fixed with 4% formaldehyde in PBS for 24 h and embedded in paraffin. Following treatments aimed to promote probe penetration and eliminate non-specific probe binding, an EGFR antisense biotin-labeled oligonucleotide probe was hybridized to sections at 45°C for 4h. High and low stringency washes were designed to remove the unbound probe. Following the blocking of non-specific tissue binding, sections were incubated with streptavidin-peroxidase. Biotinylated tyramide was then used for signal amplification. After washing, streptavidin-peroxidase was reapplied, followed by colorimetric detection of peroxidase activity using DAB as substrate. The sections were counterstained with hematoxylin and coverslipped.