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Enhancement of Differential Interference Contrast Images of Live Cells Obtained With Digital Cameras

Published online by Cambridge University Press:  02 July 2020

M.V. Parthasarathy
M.V. Parthasarathy*
Affiliation:
Plant Biology, Cornell University, Ithaca, NY14853
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Extract

The usefulness of Differential Contrast Interference (DIC) light microscopy for observing fine details within transparent specimens is well known. However, when viewed by the eye or by recording with photographic emulsion, fine structural detail at the limit of resolution is often not visible because of lack of contrast. To overcome this problem, electronic contrast enhancement capabilities of video cameras have been used to enhance structural details that would otherwise be invisible. The technique, commonly referred to as VE-DIC (Video Enhanced DIC), uses first analog contrast enhancement of the image with a video camera followed by a real-time digital image processor to further enhance the image with. We are exploring the feasibility of achieving fine structural detail of live cells by directly acquiring digital images of them with a high resolution CCD camera.

High resolution cooled slow-scan 12-bit CCD cameras are well suited for DIC microscopy because of their greater dynamic range than video CCD cameras that are normally 8-bits or lower.

Type
Biomedical Applications
Information
Microscopy and Microanalysis , Volume 5 , Issue S2: Proceedings: Microscopy & Microanalysis '99, Microscopy Society of America 57th Annual Meeting, Microbeam Analysis Society 33rd Annual Meeting, Portland, Oregon August 1-5, 1999 , August 1999 , pp. 1112 - 1113
Copyright
Copyright © Microscopy Society of America

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References

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