Hostname: page-component-586b7cd67f-dlnhk Total loading time: 0 Render date: 2024-11-24T23:24:55.200Z Has data issue: false hasContentIssue false
  • English
  • Français

Enhancement of Apoptosis-Related Gene Expression in Human Renal Graft Rejection

Published online by Cambridge University Press:  02 July 2020

P. Y. Lau ,
J. Papadimitriou ,
C. Drachenberg ,
M. R. Weir  and
C. Wei
P. Y. Lau
Affiliation:
Cardiorenal Molecular Research, Departments of Surgery, Medicine and Pathology, University of Maryland School of Medicine, Baltimore, MD, 21201
J. Papadimitriou
Affiliation:
Cardiorenal Molecular Research, Departments of Surgery, Medicine and Pathology, University of Maryland School of Medicine, Baltimore, MD, 21201
C. Drachenberg
Affiliation:
Cardiorenal Molecular Research, Departments of Surgery, Medicine and Pathology, University of Maryland School of Medicine, Baltimore, MD, 21201
M. R. Weir
Affiliation:
Cardiorenal Molecular Research, Departments of Surgery, Medicine and Pathology, University of Maryland School of Medicine, Baltimore, MD, 21201
C. Wei
Affiliation:
Cardiorenal Molecular Research, Departments of Surgery, Medicine and Pathology, University of Maryland School of Medicine, Baltimore, MD, 21201
Get access

Extract

Apoptosis or programmed cell death is involved in many diseases include end-stage renal failure. Apoptosis-related genes include both stimulate genes and inhibitory gene of apoptosis. The genes which stimulate apoptosis include p53 and p21-WAF. The genes which inhibit apoptosis include bcl-2 gene family. The mechanisms of apoptosis include p53-dependend pathway and p53- independent pathway. We hypothesized that apoptosis-related genes may activate in renal graft rejection after kidney transplantation. Therefore, the present study was designed to investigate apoptosis-related gene expression and localization by immunohistochemical staining (IHCS) in human renal tissues with graft rejection and compare with that in normal human renal tissue.

Human renal biopsy (n=5) were obtained after kidney transplantation with mild and moderate renal rejection. Normal human kidney biopsy was obtained during nephrectomy. P53, p21-WAF and Bcl-2 levels in renal tissue were determined by IHCS. The results of IHCS was evaluated by IHCS staining density scores (0, no staining; 1, minimal staining; 2, mild staining; 3, moderate staining; and 4, strong staining).

Type
Apoptosis
Information
Microscopy and Microanalysis , Volume 6 , Issue S2: Proceedings: Microscopy & Microanalysis 2000, Microscopy Society of America 58th Annual Meeting, Microbeam Analysis Society 34th Annual Meeting, Microscopical Society of Canada/Societe de Microscopie de Canada 27th Annual Meeting, Philadelphia, Pennsylvania August 13-17, 2000 , August 2000 , pp. 626 - 627
Copyright
Copyright © Microscopy Society of America

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

References:

1.Oberbauer, R., et al. J Am Soc Nephrol. (1999) 10, 2006–2013.Google Scholar
2.Chandraker, A., et al. J Clin Invest (1998) 101, 2039–2018.CrossRefGoogle Scholar
3.Wever, P. C., et al. Clin Nephrol (1998) 49, 28–34.Google Scholar
4.Ferrandiz, C., et al. Br J Dermatol (1996) 134,810–811.CrossRefGoogle Scholar
5. This research was supported in part by grants from the NIH (HL03174 & HL61299, C. Wei), AHAMD, NKF and University of Maryland School of Medicine.Google Scholar