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Endogenous IL-2 in Cancer Cells: Immunocytochemical Detection and Biological Significance

Published online by Cambridge University Press:  02 July 2020

T. L. Whiteside  and
Torsten E. Reichert
T. L. Whiteside
Affiliation:
Department of Pathology, University of Pittsburgh School of Medicine and University of Pittsburgh Cancer Institute, Pittsburgh, PA, 15213
Torsten E. Reichert
Affiliation:
Department of Pathology, University of Pittsburgh School of Medicine and University of Pittsburgh Cancer Institute, Pittsburgh, PA, 15213
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Extract

We have described previously the presence of IL2R on human carcinoma (CA) cell lines (squamous cell carcinoma of the head and neck, gastric carcinoma, renal cell carcinoma) and tumors in situ (1,2,). These carcinomas expressed IL2Rβ and γ (intermediate affinity IL2R) and I-labeled IL2 was found to bind to these receptors. Scatchard analyses showed that 13,500 IL2Rβ/cell and about 300 IL2Rα were present on the surface of PCI-1, a SCCHN cell line. The mRNA and proteins for IL2Rβ and γ were consistently detectable in tumor cell lines and fresh tumor tissues. Binding of recombinant IL2 to IL2R resulted in a partial but significant inhibition of tumor cell growth, decreased expression of class I MHC antigens and ICAM-1 on tumor cells and their increased sensitivity to growth-modulating cytokines IFN-γ, TNF-α or TGFβ. These effects were IL2-dose dependent, and were optimal at doses >10nM. Anti-IL2Rβ Abs reversed IL2-induced growth inhibition, an indication that exogenous IL2 delivered a negative growth signal to CAs via the β chain of IL2R.

Type
Neoplasia: Abnormal Cell Growth or Death/Apoptosis? Insights from Microscopy
Information
Microscopy and Microanalysis , Volume 3 , Issue S2: Proceedings: Microscopy & Microanalysis '97, Microscopy Society of America 55th Annual Meeting, Microbeam Analysis Society 31st Annual Meeting, Histochemical Society 48th Annual Meeting, Cleveland, Ohio, August 10-14, 1997 , August 1997 , pp. 9 - 10
Copyright
Copyright © Microscopy Society of America 1997

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References

Refernces

1.Weidman, E.et al., Receptors for interleukin 2 on human squamous cell carcinoma cell lines and tumor in situ. Cancer Res., 52:5963–5970,1992.Google Scholar
2.Yasumura, S.et al., Expression of interleukin 2 receptors on human carcinoma cell lines and tumor growth inhibition by interleukin 2. Int. J. Cancer. 58:1–10, 1994.Google Scholar
3.Lin, W-c, Yasumura, S., and Whiteside, T.L.Transfer of interleukin 2 receptor genes into squamous cell carcinoma: Modification of tumor cell growth. Arch. Otolaryngol. Head Neck Surg., 119:1229–1235, 1993.10.1001/archotol.1993.01880230075012CrossRefGoogle ScholarPubMed
4.Lin, W-c.,. Constitutive production of interleukin 2 by human carcinoma cells, expression of IL2R and tumor cell growth. J. Immunol, 155:4805–4816, 1995.Google ScholarPubMed
5.Reichert, T.E.et al., Interleukin 2 expression in human carcinoma cell lines and its role in cell cycle progression Submitted, 1997.Google Scholar