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Electrostatic Capture Following Laser Microdissection for the Preparation of Homogeneous Biological Specimens

Published online by Cambridge University Press:  28 November 2016

Dingrong Yi ,
Linghua Kong ,
Ranjith K. Kankala  and
Zi Wang
Dingrong Yi*
Affiliation:
College of Mechanical Engineering and Automation, Huaqiao University, No. 668 Jimei Road, Jimei District, Xiamen, Fujian Province 361021, P. R. China
Linghua Kong
Affiliation:
College of Mechanical Engineering and Automation, Huaqiao University, No. 668 Jimei Road, Jimei District, Xiamen, Fujian Province 361021, P. R. China
Ranjith K. Kankala
Affiliation:
College of Chemical Engineering, Huaqiao University, No. 668 Jimei Road, Jimei District, Xiamen, Fujian Province 361021, P. R. China
Zi Wang
Affiliation:
College of Mechanical Engineering and Automation, Huaqiao University, No. 668 Jimei Road, Jimei District, Xiamen, Fujian Province 361021, P. R. China
*
*Corresponding author. [email protected]
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Abstract

There is an unmet need for researchers in life sciences and clinical pathology to obtain untainted target cells with very high accuracy, which are suitable for subsequent genome and protein analysis. In this paper, an electrostatic capture laser microdissection technology (ECM) is proposed and explained. Following microscopic identification and separation of target cells from the surrounding tissues using laser cutting, the ECM uses electrostatic forces to capture target cells. Validation experiments indicate that ECM can capture a wide assortment of contamination-free homogeneous samples, ranging from very tiny pieces of a few micrometers in diameter to large pieces with a surface area of over 40,000 µm2. Evidence is also provided indicating that uncontaminated homogeneous tissue materials collected by ECM are suitable for further DNA and RNA analysis. This suggests that ECM capture causes little or no identifiable damage to the collected tissues. This technique has significant advantages compared with existing traditional capture methods, such as very low risk of biological sample damage and the fact that it can be applied to both upright and inverted microscopy. The latter allows for separating target cells in tissue culture. ECM usage provides a cost-effective alternative to more traditional laser capture microdissection techniques.

Keywords

electrostatic captureelectrostatic forcecontact-freecontamination-freelaser micro-dissection
Type
Instrumentation and Software Techniques
Information
Microscopy and Microanalysis , Volume 22 , Issue 6 , December 2016 , pp. 1329 - 1337
Copyright
© Microscopy Society of America 2016 

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References

Almeida, M.R.D. & Strömvik, M.V. (2016). Laser capture microdissection: Avoiding bias in analysis by selecting just what matters. Biotechnology of Plant Secondary Metabolism 1405, 109–119.Google Scholar
Amado, R.G., Wolf, M., Peeters, M., Van Cutsem, E., Siena, S., Freeman, D.J., Juan, T., Sikorski, R., Suggs, S., Radinsky, R., Patterson, S.D. & Chang, D.D. (2008). Wild-type KRAS is required for panitumumab efficacy in patients with metastatic colorectal cancer. J Clin Oncol 31(26), 5139.Google Scholar
Arcturus BioScience, Inc. (2016). Optimized protocol for mounting tissue sections onto metal-framed PEN membrane slides. Available at http://www.bmbio.com/file/201758.pdf (retrieved 19 February 2016).Google Scholar
Baer, T.M.E.M. (1998). Laser capture micro-dissection method and apparatus. E. Patent. EP 0958491 B1.Google Scholar
Böhm, M.S.C.W. (1999). Membrane-based laser micro-dissection in molecular oncology. Onkologie 22, 296–301.Google Scholar
Burgemeister, R. (2004). New aspects of laser microdissection in research and routine. J Histochem Cytochem 53(3), 4.Google Scholar
Chen, C.M., Lee, J.A., & Yen, C.F. (2009). Improvement in resolution of laser capture microdissection using near-field probe to capture nanoparticles. IEEE Trans Nanobioscience 8(2), 113–119.CrossRefGoogle ScholarPubMed
Cheng, L., Zhang, S., Maclennan, G.T, Williamson, S.R., Davidson, D.D., Wang, M., Jones, T.D., Lopezbeltran, A. & Montironi, R. (2012). Laser-assisted microdissection in translational research: Theory, technical considerations, and future applications. Appl Immunohistochem Mol Morphol 21(1), 31–47.Google Scholar
Datta, S., Malhotra, L., Dickerson, R., Chaffee, S., Sen, C.K. & Roy, S. (2015). Laser capture microdissection: Big data from small samples. Histol Histopathol 30, 1255–1269.Google Scholar
Decarlo, K., Emley, A., Dadzie, O.E. & Mahalingam, M. (2011). Laser capture microdissection: Methods and applications. Methods Mol Biol 755, 1–15.Google Scholar
Emmert-Buck, M.R.B.R. (1996). Laser capture micro-dissection. Science 274(5289), 998–1001.CrossRefGoogle Scholar
Espina, V.W.J.D. (2006). Laser-capture microdissection. Nat Protoc 1, 586–603.Google Scholar
Fink, L.S.W.E. (1998). Real-time quantitative RT-PCR after laser-assisted cell picking. Nat Med 4(11), 1329–1333.Google Scholar
Jiang, R., Lu, Y.T., Hao, H., Li, B., Chen, J.F., Lin, M., Li, F., Wu, K., Wu, H. & Lichterman, J. (2015). A comparison of isolated circulating tumor cells and tissue biopsies using whole-genome sequencing in prostate cancer. Oncotarget 6(42), 44781–44793.Google Scholar
Karapetis, C.S., Khambata-Ford, S., Jonker, D.J., O'Callaghan, C.J., Tu, D., Tebbutt, N.C., Simes, R.J., Chalchal, H., Shapiro, J.D., Robitaille, S., Price, T.J., Shepherd, L., Au, H.-J., Langer, C., Moore, M.J. & Zalcberg, J.R. (2008). K-ras mutations and benefit from cetuximab in advanced colorectal cancer. N Engl J Med 17(359), 1757–1765.Google Scholar
Kolble, K. (2000). The Leica micro-dissection system: design and applications. J Mol Med 78(7), B24–B25.Google Scholar
Lee, J.Y.D.S. (1998). A simple, precise and economical microdissection technique for analysis of genomic DNA from archival tissue sections. Virchows Archiv 433(4), 5.Google Scholar
Leica Microsystems (2012). Sample preparation for Leica laser microdissection. Protocol guide for Leica microsystems laser microdissection systems. Available at http://www.unige.ch/medecine/bioimaging/equipment/microscopes/protocoles.pdf. Google Scholar
Markman, M.W.M.L. (2014). Colorectal cancer and KRAS/SBRAF. Medscape http://emedicine.medscape.com/article/1690010-overview.Google Scholar
Murray, G.I. & Curran, S. (2005). Laser capture micro-dissection.New York: Humana Press.Google Scholar
Okuducu, A.F.H.J. (2005). Laser-assisted microdissection, techniques and applications in pathology (review). Int J Mol Med 15(5), 763–769.Google Scholar
Rabien, A. & Kristiansen, G. (2016). Tissue microdissection. Cancer Gene Profiling 1381, 39–52.Google Scholar
Rodríguez-Perálvarez, M., Vinh Luong, T., Lorenzo Andreana, M.D., Meyer, T., Frcp, A.P.D. & Fmedsci, A.K.B. (2012). A systematic review of microvascular invasion in hepatocellular carcinoma: Diagnostic and prognostic variability. Ann Surg Oncol 20(1), 325–339.Google Scholar
Satori, C.P., Kostal, V. & Arriaga, E.A. (2012). Review on recent advances in the analysis of isolated organelles. Anal Chim Acta 753(21), 8–18.Google Scholar
Schutze, R. & Schutze, K. (1997). Method and device for the contactless laser assisted microinjection, sorting and production of biological objects generated in a planar manner. United States Patent 5998129, USA and PALM Gmbh, Silicon Valley.Google Scholar
Schutze, K. & Lahr, G. (1998). Identification of expressed genes by laser-mediated manipulation of single cells. Nat Biotechnol 16(8), 737–742.Google Scholar
Schutze, K., Pos, H. & Lahr, G. (1998). Laser micromanipulation systems as universal tools in cellular and molecular biology and in medicine. Mol Cell Biol 44(5), 735–746.Google Scholar
Sook, H.C. & Weiyong, S. (2015). Laser capture microdissection: from its principle to applications in research on neurodegeneration. Neural Regen Res 10(6), 897–898.Google Scholar
Sturm, D., Marselli, L., Ehehalt, F., Richter, D., Distler, M., Kersting, S., Grützmann, R., Bokvist, K., Froguel, P., Liechti, R., Jörns, A., Meda, P., Baretton, G.B., Saeger, H., Schulte, A.M., Marchetti, P. & Solimena, M. (2013). Improved protocol for laser micro-dissection of human pancreatic islets from surgical specimens. J Vis Exp 6(71), e50231-e50231.Google Scholar
Whetsell, L.M.G.N. (1992). Polymerase chain reaction microanalysis of tumors from stained histological slides. Oncogene 7(11), 2355–2361.Google ScholarPubMed
Yi, D. (2012). A device, method and system for collecting cells after separated by a laser micro-dissection system. CN201210052939.3.Google Scholar
Zhuang, Z.B.P.E. (1995). A microdissection technique for archival DNA analysis of specific cell populations in lesions <1 mm in size. Am J Pathol 146(3), 620–625.Google Scholar