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Effects of Varying Salinity on the Endoplasmic Reticulum System in Leaf Epidermal Cells of Ruppia Maritima

Published online by Cambridge University Press:  02 July 2020

A.D. Barnabas
A.D. Barnabas*
Affiliation:
Department of Botany, University of Durban-Westville, Private Bag X54001, Durban4000, South Africa
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Extract

Ruppia maritima L. is a submerged halophyte which is capable of growing in a wide range of salinities. In a previous study, the effects of varying salinity on the ultrastructure of leaf blade epidermal cells of R. maritima were investigated. The ultrastructural morphology of the cells, including organelle number and distribution, differed at different salinities. However, salinity effects on the endoplasmic reticulum (ER) system could not be assessed since this cytoplasmic component was not readily discernible in material processed in the conventional manner for transmission electron microscopy (TEM). In the present study, selective staining of the ER was achieved, and the response of this membrane system to different salinities ascertained.

Leaf material from plants growing at two salinities (5‰and 20‰) was examined. The zinc iodide-osmium tetroxide (ZIO) impregnation technique was used to stain the ER, and its morphology and distribution determined in leaf blade epidermal cells exposed to the different salinities. Leaf samples were fixed in glutaraldehyde, post fixed in an aqueous mixture of ZIO, dehydrated, and embedded in resin. Controls were processed in exactly the same way, but without ZIO post fixation. Sections with dark gold to purple interference colours were cut and examined unstained with a Phillips 301 TEM.

Type
Plant Biology and Pathology
Information
Microscopy and Microanalysis , Volume 3 , Issue S2: Proceedings: Microscopy & Microanalysis '97, Microscopy Society of America 55th Annual Meeting, Microbeam Analysis Society 31st Annual Meeting, Histochemical Society 48th Annual Meeting, Cleveland, Ohio, August 10-14, 1997 , August 1997 , pp. 97 - 98
Copyright
Copyright © Microscopy Society of America 1997

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References

1.Jagels, R. and Barnabas, A.D., Aquat. Bot. 33 (1989) 207.10.1016/0304-3770(89)90038-7CrossRefGoogle Scholar
2.Harris, N, Planta 146 (1979) 63.10.1007/BF00381256CrossRefGoogle Scholar
3. The author gratefully acknowledges the financial support of the University of Durban-Westville and the Foundation for Research and Development (FRD), South Africa.Google Scholar