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Effects of Pamidronate on Dental Enamel Formation Assessed by Light Microscopy, Energy-Dispersive X-Ray Analysis, Scanning Electron Microscopy, and Microhardness Testing

Published online by Cambridge University Press:  23 May 2016

Ana P. Soares ,
Renan F. do Espírito Santo ,
Sérgio R. P. Line ,
Maria das G. F. Pinto ,
Pablo de M. Santos ,
Maria Betania P. Toralles  and
Alexandre R. do Espírito Santo
Ana P. Soares
Affiliation:
Multidisciplinary Institute of Health, Federal University of Bahia—UFBA, Rua Rio de Contas, 58, Quadra 17, Lote 58, Candeias, Vitória da Conquista, BA CEP 45.029-094, Brasil
Renan F. do Espírito Santo
Affiliation:
Faculty of Pharmacy, Federal University of Bahia—UFBA, Rua Barão de Jeremoabo, S/N, Campus Universitário de Ondina, Ondina, Salvador, BA CEP 40.170-115, Brasil
Sérgio R. P. Line
Affiliation:
Piracicaba Dental School, University of Campinas—UNICAMP, Av. Limeira, 901, Areião, Piracicaba, SP CEP 13.414-903, Brasil
Maria das G. F. Pinto
Affiliation:
School of Veterinary Medicine and Zootechny, Federal University of Bahia—UFBA, Av. Ademar de Barros, 500, Ondina, Salvador, BA CEP 40.170-110, Brasil
Pablo de M. Santos
Affiliation:
Professor Edgard Santos University Hospital, Federal University of Bahia—UFBA, Rua Augusto Viana, S/N, Canela, Salvador, BA CEP 40.110-060, Brasil
Maria Betania P. Toralles
Affiliation:
Institute of Health Sciences, Federal University of Bahia—UFBA, Av. Reitor Miguel Calmon, S/N, Vale do Canela, Salvador, BA CEP 40.110-902, Brasil
Alexandre R. do Espírito Santo*
Affiliation:
Institute of Health Sciences, Federal University of Bahia—UFBA, Av. Reitor Miguel Calmon, S/N, Vale do Canela, Salvador, BA CEP 40.110-902, Brasil
*
*Corresponding author. [email protected]
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Abstract

The aim of the present work was to investigate birefringence and morphology of the secretory-stage enamel organic extracellular matrix (EOECM), and structural and mechanical properties of mature enamel of upper incisors from adult rats that had been treated with pamidronate disodium (0.5 mg/kg/week for 56 days), using transmitted polarizing and bright-field light microscopies (TPLM and BFLM), energy-dispersive X-ray (EDX) analysis, scanning electron microscopy (SEM) and microhardness testing. BFLM showed no morphological changes of the EOECM in pamidronate and control groups, but TPLM revealed a statistically significant reduction in optical retardation values of birefringence brightness of pamidronate-treated rats when compared with control animals (p<0.01). EDX analysis showed that pamidronate-induced statistically significant decrease in phosphorus’ quantity in outer mature enamel (p<0.01) and an increase in the calcium/phosphorus ratio in that structure (p<0.05). Pamidronate did not induce ultra-structural alterations in mature enamel as revealed by SEM and did not cause a reduction in its microhardness (p>0.05). The present study indicates that pamidronate can affect birefringence of the secretory-stage EOECM, which does not seem to be associated with significant changes in morphological and/or mechanical properties of mature enamel.

Keywords

bisphosphonatesenamellight microscopyscanning electron microscopymicroanalysis
Type
Biological Applications
Information
Microscopy and Microanalysis , Volume 22 , Issue 3 , June 2016 , pp. 640 - 648
Copyright
Copyright © Microscopy Society of America 2016

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