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Effectiveness Of Immunolabeling Gfap For Estimating Astrocytic Shape And Volume

Published online by Cambridge University Press:  02 July 2020

E. Bushong ,
M. E. Martone ,
C. Foster  and
M. H. Ellisman
E. Bushong
Affiliation:
Biomedical Sciences Program
M. E. Martone
Affiliation:
Department of Neurosciences and National Center for Microscopy and Imaging Research University of California, , San Diego, 9500 Gilman Dr., La Jolla, CA92093-0608
C. Foster
Affiliation:
Department of Neurosciences and National Center for Microscopy and Imaging Research University of California, , San Diego, 9500 Gilman Dr., La Jolla, CA92093-0608
M. H. Ellisman
Affiliation:
Department of Neurosciences and National Center for Microscopy and Imaging Research University of California, , San Diego, 9500 Gilman Dr., La Jolla, CA92093-0608
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Extract

Each astrocyte forms an extensive network of fine processes within the surrounding neural tissue, interacting extensively with neighboring neurons and blood vessels. Fine glial processes surround synapses and probably modulate synaptic transmission. Glial endfeet on capillaries are responsible for transport of ions and metabolites and possibly control blood flow. Alterations in these fine structures may be of significance in brain function and disease. Glial fibrillary acidic protein (GFAP) is an intermediate filament found in astrocytes of the central nervous system. GFAP is commonly found in the perikarya and processes of protoplasmic and fibrous type astrocytes. Immunohistochemical labeling of GFAP is extensively used as a means of determining the location and shape of astrocytes. However, its labeling pattern varies with brain region (e.g. cortex vs. hippocampus), with cell state (natural vs. reactive astrocytes), and with the specific α- GFAP antibody used. Furthermore, Golgi-stained or dye-filled astrocytes show numerous small appendages or vellate structures that conform to the surrounding tissue and do not stain for GFAP.

Type
Cytochemistry
Information
Microscopy and Microanalysis , Volume 5 , Issue S2: Proceedings: Microscopy & Microanalysis '99, Microscopy Society of America 57th Annual Meeting, Microbeam Analysis Society 33rd Annual Meeting, Portland, Oregon August 1-5, 1999 , August 1999 , pp. 1340 - 1341
Copyright
Copyright © Microscopy Society of America

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References

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