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The Effect of Tgfß-2 on Inner Ear Development: Light and Electron Microscopy Observations

Published online by Cambridge University Press:  02 July 2020

R. Friedman ,
N. Paradies ,
S. Wert ,
T. Doetschman  and
E.L. Cardell
R. Friedman
Affiliation:
Department of Otolaryngology University of Cincinnati College of Medicine, Cincinnati, OH45267 Department of Cell Biology, Neurobiology and Anatomy University of Cincinnati College of Medicine, CincinnatiOH, 45267
N. Paradies
Affiliation:
Department of Otolaryngology University of Cincinnati College of Medicine, Cincinnati, OH45267
S. Wert
Affiliation:
Department of Pediatrics University of Cincinnati College of Medicine, CincinnatiOH, 45267
T. Doetschman
Affiliation:
Department of Molecular Genetics, Biochemistry and Microbiology, and University of Cincinnati College of Medicine, CincinnatiOH, 45267
E.L. Cardell
Affiliation:
Department of Cell Biology, Neurobiology and Anatomy University of Cincinnati College of Medicine, CincinnatiOH, 45267
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Extract

Transforming growth factor beta (TGFß) genes are linked to a variety of developmental processes and are the subject of in vivo and in vitro transgene research studies. We are evaluating TGFß-2 effects on mouse inner ear development, with emphasis on the cochlear duct (CD), by comparing plastic sections of intact inner ears from developmental day (D) 16.5,18.5 and 19.5 littermates with wildtype (+/+), heterozygous (+/−) and mutant (−/−) TGFß-2 genotypes as determined by polymerase chain reaction analysis of tail digests. Auditory and vestibular organs of all D16.5 mice appear similar: membranous labyrinth epithelium varies from simple cuboidal/low columnar to pseudostratified/stratified columnar. Surrounding mesenchyme varies in cell density regionally, the most cellular mesenchyme underlies areas of sensory epithelium. Sparse mesenchymal cell distribution in the vestibule and basal CD indicates sites of perilymph channel formation. The spiral and vestibular ganglia and their unmyelinated fibers are prominent. Otoconia and hair cells are present in the utricle (U) and saccule (S) maculae; hair cells are less easily identifiable in the CD.

Type
Developmental and Reproductive Biology
Information
Microscopy and Microanalysis , Volume 3 , Issue S2: Proceedings: Microscopy & Microanalysis '97, Microscopy Society of America 55th Annual Meeting, Microbeam Analysis Society 31st Annual Meeting, Histochemical Society 48th Annual Meeting, Cleveland, Ohio, August 10-14, 1997 , August 1997 , pp. 173 - 174
Copyright
Copyright © Microscopy Society of America 1997

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References

1. R.W., Peltonet al., J Cell Biol. (1991);115: 1091Google Scholar

2. The authors gratefully acknowledge the skilled assistance of Long, D. and Esmahan, P. in preparing text and photographsGoogle Scholar

3. This research is supported by NIH Grant #K08DC00119-01Al to Friedman, R. and NIH Grant #R01-HD26471-06 to Doetschman, T.Google Scholar