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Effect of Sublethal Nickel Chloride Exposure on Crayfish, Astacus leptodactylus Ovary: An Ultrastructural, Autometallographic, and Electrophoretic Analyses

Part of: Micrographia Collection

Published online by Cambridge University Press:  10 May 2017

Otilia Zarnescu ,
Ana-Maria Petrescu ,
Alexandra Gaspar  and
Oana Craciunescu
Otilia Zarnescu*
Affiliation:
Laboratory of Histology and Developmental Biology, Faculty of Biology, University of Bucharest, Splaiul Independentei 91-95, Bucharest, R-050095, Romania
Ana-Maria Petrescu
Affiliation:
Laboratory of Histology and Developmental Biology, Faculty of Biology, University of Bucharest, Splaiul Independentei 91-95, Bucharest, R-050095, Romania “Grigore Antipa” National Museum of Natural History, Kiseleff 1, Bucharest, R-011341, Romania
Alexandra Gaspar
Affiliation:
Department of Cellular and Molecular Biology, National Institute of Research and Development for Biological Sciences, Splaiul Independentei 296, Bucharest, R-060031, Romania
Oana Craciunescu
Affiliation:
Department of Cellular and Molecular Biology, National Institute of Research and Development for Biological Sciences, Splaiul Independentei 296, Bucharest, R-060031, Romania
*
*Corresponding author. [email protected]
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Abstract

Cytological responses in different organs of sentinel organisms have proven to be useful tools for characterizing the health status of those organisms and assessing the impact of environmental contaminants. Our study shows that nickel (II) accumulated in both germ cells (oogonia and developing oocytes) and somatic cells (muscle cells, follicle cells) in the Astacus leptodactylus ovary. Muscle cells from ovarian wall show disorganization and the disruption of cytoplasmic microtubules and pyknosis of the cell nucleus. Follicle cells, both those that surround the developing oocytes and also those that are not associated with the oocytes contained within the cytoplasm vacuoles of different sizes, degenerated mitochondria, myelin bodies, disorganized microtubules, and pyknotic nuclei. The most evident pathological phenomenon was the alteration and disorganization of the basal matrix, which separates the ovarian interstitium from ovarian follicles compartment. Exposure to nickel induces cytoplasmic vacuolation in oogonia and developing oocytes, structural alteration of the developing yolk granules and condensation of the nucleoli. Ultrastructural autometallography has shown grains of silver-enhanced nickel inside the cytoplasm of the muscle cells with altered morphology, including the cytoplasm, nucleus, and basal matrix of the follicle cells, and in intracisternal granules and developing yolk granules of the oocytes.

Keywords

crayfishovarynickelultrastructureautometallography
Type
Micrographia
Information
Microscopy and Microanalysis , Volume 23 , Issue 3 , June 2017 , pp. 668 - 678
Copyright
© Microscopy Society of America 2017 

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