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EDS of thin Biological Specimen in the Study of Time-Dependent Physiological Processes

Published online by Cambridge University Press:  02 July 2020

M.F. Wendt-Gallitelli ,
Tilman Voigt ,
Michael Schultz ,
Frank Rudolf  and
Gerrit Isenberg
M.F. Wendt-Gallitelli
Affiliation:
Department of Physiology, University of Halle, MagdeburgerStr.6, D-06097, Germany
Tilman Voigt
Affiliation:
Department of Physiology, University of Tubingen, Gmelin Str.5, D-72076
Michael Schultz
Affiliation:
Department of Physiology, University of Halle, MagdeburgerStr.6, D-06097, Germany
Frank Rudolf
Affiliation:
Department of Physiology, University of Halle, MagdeburgerStr.6, D-06097, Germany
Gerrit Isenberg
Affiliation:
Department of Physiology, University of Halle, MagdeburgerStr.6, D-06097, Germany
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Extract

The contraction of muscle depends on the ability of intracellular organelles to rapidly release and take up large amounts of activator calcium. In cardiac muscle the intracellular sodium concentration is known to also modulate this Ca2+ release: Na+-Ca2+ exchange creates narrow cytosolic microdomains just internal to the sarcolemma (approximately 20 nm wide) with locally elevated concentrations of Ca2+ and Na+. The existence of such “functional” compartments with transient high ionic concentrations has been postulated for explaining the nigh efficiency of signal transmission during excitation-contraction coupling. We present results showing that electron probe microanalysis (EPMA) at the high spatial resolution of scanning transmission electron microscopy (STEM) can detect changes in sodium and calcium concentration of membrane-limited organelles and functional microdomains provided the cardiac myocytes are rapidly frozen at defined times during activation of contraction.

Type
30 Years of Energy Dispersive Spectrometry in Microanalysis
Information
Microscopy and Microanalysis , Volume 4 , Issue S2: Proceedings: Microscopy & Microanalysis '98, Microscopy Society of America 56th Annual Meeting, Microbeam Analysis Society 32nd Annual Meeting, Atlanta, Georgia July 12-16, 1998 , July 1998 , pp. 192 - 193
Copyright
Copyright © Microscopy Society of America

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References

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8. Supported by the Deutsche Forschungsgemeinschaft, Grant We 879/5-1 and BMBF 313005 to M.F.W.G.Google Scholar