Hostname: page-component-cd9895bd7-dk4vv Total loading time: 0 Render date: 2024-12-26T07:04:09.283Z Has data issue: false hasContentIssue false
  • English
  • Français

Dynamics Of Mitochondria In ρ° Human Cells Repopulated With Exogenous Mtdna Observed By Transmission Electron Microscopy

Published online by Cambridge University Press:  02 July 2020

Yun Lu  and
Michael P. King
Yun Lu
Affiliation:
Neurology Department, College of Physicians and Surgeons, Columbia University & Columbia Presbyterian Medical Center, 163 W168th St., New York, NY10032
Michael P. King
Affiliation:
Neurology Department, College of Physicians and Surgeons, Columbia University & Columbia Presbyterian Medical Center, 163 W168th St., New York, NY10032
Get access

Extract

Mitochondrial DNA (mtDNA) mutations have been found associated with several neuromuscular diseases. In order to address further mechanisms of pathogenesis of the specific mtDNA mutations in responsible for these diseases, the ρ° human cell culture system has been established and has been widely used in recent years (4)'(5). This system is based upon the repopulating of human cells, which are completely depleted of mtDNA (termed ρ° cells), with exogenous mitochondria containing the mtDNA of interest. Molecular genetic and biochemical analysis showed that transformants can be obtained with various mitochondrial donors and the respiratory competence can be restored. In this study, the ultrastructural and immunocytochemical variations of mitochondria in human cell lines cultured in this system observed by transmission electron microscopy are reported.

Cell cultures for wild-type 143B, ρ206, and transmitochondrial cell lines have been described previously. Cells were trypsinized and collected from individual culture plates, and then were fixed with 3% paraformaldehyde and 0.04% glutaradehyde in 0.1 M phosphate-buffered saline (PBS) with 4% sucrose (pH 7.6) at RT.

Type
Biological Ultrastructure (Cells, Tissues, Organ Systems)
Information
Microscopy and Microanalysis , Volume 5 , Issue S2: Proceedings: Microscopy & Microanalysis '99, Microscopy Society of America 57th Annual Meeting, Microbeam Analysis Society 33rd Annual Meeting, Portland, Oregon August 1-5, 1999 , August 1999 , pp. 1272 - 1273
Copyright
Copyright © Microscopy Society of America

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

(1)HOLT, I.J., etal. Nature 331, 717 (1988)CrossRefGoogle Scholar
(2)Zeviani, M., et al, Neurology 38, 1339 (1988)CrossRefGoogle Scholar
(3)Wallace, D. C., et al, Science 242, 1427(1988)CrossRefGoogle Scholar
(4)King, M. P. & Attardi, G., Science 246, 500 (1989)CrossRefGoogle Scholar
(5)King, M. P.. et al, Mol. Cell Biol. 12, 480 (1992)CrossRefGoogle Scholar
(6)Berryman, M. A. & Rodewald, R. D., J. Histochem. Cytochem 38, 159, (1990)CrossRefGoogle Scholar