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Distribution of Lignin, Hemicellulose, and Arabinogalactan Protein in Hemp Phloem Fibers

Part of: Micrographia Collection

Published online by Cambridge University Press:  03 September 2018

Shingo Kiyoto ,
Arata Yoshinaga ,
Eva Fernandez-Tendero ,
Arnaud Day ,
Brigitte Chabbert  and
Keiji Takabe
Shingo Kiyoto*
Affiliation:
Research Institute for Sustainable Humanosphere (RISH), Kyoto University, Uji, Kyoto 611-0011, Japan
Arata Yoshinaga
Affiliation:
Laboratory of Tree Cell Biology, Division of Forest and Biomaterials Science, Graduate School of Agriculture, Kyoto University, Sakyo-ku, Kyoto 606-8502, Japan
Eva Fernandez-Tendero
Affiliation:
FARE Laboratory, INRA, Université de Reims Champagne-Ardenne, 51100 Reims, France Fibres Recherche Développement, Technopole de l’Aube en Champagne – Hôtel de Bureaux 2, 2 rue Gustave Eiffel, CS 90601, 10901 TROYES, Cedex 9, France CNRS, UMR8576, UGSF- Unité de Glycobiologie Structurale et Fonctionnelle, Unité de Lille, 59000 Lille, France
Arnaud Day
Affiliation:
Fibres Recherche Développement, Technopole de l’Aube en Champagne – Hôtel de Bureaux 2, 2 rue Gustave Eiffel, CS 90601, 10901 TROYES, Cedex 9, France CNRS, UMR8576, UGSF- Unité de Glycobiologie Structurale et Fonctionnelle, Unité de Lille, 59000 Lille, France
Brigitte Chabbert
Affiliation:
FARE Laboratory, INRA, Université de Reims Champagne-Ardenne, 51100 Reims, France
Keiji Takabe
Affiliation:
Laboratory of Tree Cell Biology, Division of Forest and Biomaterials Science, Graduate School of Agriculture, Kyoto University, Sakyo-ku, Kyoto 606-8502, Japan
*
*Author for correspondence: Shingo Kiyoto, E-mail: [email protected]
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Abstract

The distribution of lignin, 8-5′ and 8-8′ linked lignin substructure, and noncellulosic polysaccharides in hemp (Cannabis sativa L.) phloem fibers were explored based on histochemical and immunological methods. Ultraviolet absorption and potassium permanganate staining were observed mainly in the compound middle lamella (CML) and S1 layers, and rarely in the G-layer of phloem fibers, suggesting that lignin concentration is high at the CML and S1 layers, and very low at the G-layer of hemp fibers. Acriflavine staining, uniform KM1 labeling (8-5′ linked lignin substructure), and no KM2 labeling (8-8′ linked structure) were observed in the G-layer, suggesting that there is a small amount of lignin-like compound with 8-5′ linked structure in the G-layer. In addition, some fiber cells showed a multilayered structure. Uniform arabinogalactan protein (AGP) labeling was observed on the S1 layers and G-layers using JIM14, but little appeared in the CML of hemp fibers, indicating that these layers of the phloem fibers contain AGP. Immunogold labeling of xylan (LM11) and glucomannan (LM21) showed that xylan and glucomannan were mainly present in the S1 layers and the G-layers, respectively. In some phloem fibers, LM21 immunofluorescence labeling showed multilayered structure, suggesting the heterogeneous distribution of glucomannan.

Keywords

Cannabis sativa L.lignin8-5′ linkage8-8′ linkagemonoclonal antibody
Type
Micrographia
Information
Microscopy and Microanalysis , Volume 24 , Issue 4 , August 2018 , pp. 442 - 452
Copyright
© Microscopy Society of America 2018 

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References

Abot, A, Bonnafoud, C, Touchard, F, Thibault, F, Chocinski-Arnault, L, Lemoine, R Dédaldéchamp, F (2013) Effects of cultural conditions on the hemp (Cannabis sativa) phloem fibres: Biological development and mechanical properties. J Comp Mater 47, 1067–1077.Google Scholar
Andéme-Onzighi, C, Girault, R, His, I, Morvan, C Driouich, A (2000) Immunocytochemical characterization of early-developing flax fiber cell walls. Protoplasma 213, 235–245.Google Scholar
Behr, M, Legay, S, Zizkova, E, Motyka, V, Dobrev, PI, Hausman, J-F, Lutts, S Guerriero, G (2016) Studying secondary growth and bast fiber development: The hemp hypocotyl peeks behind the wall. Front Plant Sci 7, 1733.Google Scholar
Bennett, SJ, Snell, R Wright, D (2006) Effect of variety, seed rate and time of cutting on fibre yield of dew-retted hemp. Ind Crops Prod 24, 79–86.Google Scholar
Blake, AW, Marcus, SE, Copeland, JE, Blackburn, RS Knox, JP (2008) In situ analysis of cell wall polymers associated with phloem fibre cells in stems of hemp, Cannabis sativa L. Planta 228, 1–13.Google Scholar
Bland, DE, Foster, RC Logan, AF (1971) The mechanism of permanganate and osmium tetroxide fixation and the distribution of lignin in the cell wall of Pinus radiata . Holzforschung 25, 137–143.Google Scholar
Bonatti, PM, Ferrari, C, Focher, B, Grippo, C, Torri, G Cosentino, C (2004) Histochemical and supramolecular studies in determining quality of hemp fibres for textile applications. Euphytica 140, 55–64.Google Scholar
Chantreau, M, Portelette, A, Dauwe, R, Kiyoto, S, Crônier, D, Morreel, K, Arribat, S, Neutelings, G, Chabi, M, Boerjan, W, Yoshinaga, A, Mesnard, F, Grec, S, Chabbert, B Hawkins, S (2014) Ectopic lignification in the flax lignified bast fiber1 mutant stem is associated with tissue-specific modifications in gene expression and cell wall composition. Plant Cell 26, 4462–4482.Google Scholar
Crônier, D, Monties, B Chabbert, B (2005) Structure and chemical composition of bast fibers isolated from developing hemp stem. J Agric Food Chem 53, 8279–8289.Google Scholar
Davis, EA, Derouet, C, Herve, DPC Morvan, C (1990) Isolation and an NMR study of pectins from flax (Linum usitatissimum L.). Carbohydr Res 197, 205–215.Google Scholar
Day, A, Ruel, K, Neutelings, G, Crônier, D, David, H, Hawkins, S Chabbert, B (2005) Lignification in the flax stem: Evidence for an unusual lignin in bast fibers. Planta 222, 234–245.Google Scholar
del Río, JC, Rencoret, J, Gutierrez, A, Nieto, L, Jimenez-Barbero, J Martínez, AT (2011) Structural characterization of guaiacyl-rich lignins in flax (Linum usitatissimum) fibers and shives. J Agric Food Chem 59, 11088–11099.Google Scholar
Donaldson, LA, Hague, J Snell, R (2001) Lignin distribution in coppice poplar, linseed and white straw. Holzforschung 55, 379–385.Google Scholar
Fernandez-Tendero, E, Day, A, Legros, S, Habrant, A, Hawkins, S Chabbert, B (2017) Changes in hemp secondary fiber production related to technical fiber variability revealed by light microscopy and attenuated total reflectance Fourier transform infrared spectroscopy. Plos One 12(6), e0179794.Google Scholar
Girault, R, Bert, F, Rihouey, C, Jauneau, A, Morvan, C Jarvis, M (1997) Galactans and cellulose in flax fibres: Putative contributions to the tensile strength. Int J Biol Macromol 21, 179–188.Google Scholar
Gorshkova, T, Brutch, N, Chabbert, B, Deyholos, M, Hayashi, T, Lev-Yadun, SJ, Mellerowicz, E, Morvan, C, Neutelings, G Pilate, G (2012) Plant fiber formation: State of the art, recent and expected progress, and open questions. Crit Rev Plant Sci 31, 201–228.Google Scholar
Gorshkova, T Morvan, C (2006) Secondary cell-wall assembly in flax phloem fibres: Role of galactans. Planta 223, 149–158.Google Scholar
Gorshkova, TA, Chemikosova, SB, Sal’nikov, V, Pavlencheva, NV, Gur’janov, OP, Stolle-Smits, T van Dam, JEG (2004) Occurrence of cell-specific galactan is coinciding with bast fiber developmental transition in flax. Ind Crops Prod 19, 217–224.Google Scholar
Hoffmann, P Parameswaran, N (1976) On the ultrastructural localization of hemicelluloses within delignified tracheids of spruce. Holzforschung 30, 62–70.Google Scholar
Joseleau, JP, Faix, O, Kuroda, K Ruel, K (2004a) A polyclonal antibody directed against syringyl propane epitopes of native lignins. C R Biol 327, 809–815.Google Scholar
Joseleau, JP, Imai, T, Kuroda, K Ruel, K (2004b) Detection in situ and characterization of lignin in the G-layer of tension wood fibres of Populus deltoides . Planta 219, 338–345.Google Scholar
Joseleau, JP Ruel, K (1997) Study of lignification by non invasive techniques in growing maize internodes–An investigation by Fourier transform infrared, cross-polarisation-magic angle spinning 13C-nuclear magnetic resonance spectroscopy and immunocytochemical transmission electron microscopy. Plant Physiol 114, 1123–1133.Google Scholar
Kiyoto, S, Yoshinaga, A Takabe, K (2013) Immunolocalization of 8-5’ and 8-8’ linked structures of lignin in cell walls of Chamaecyparis obtusa using monoclonal antibodies. Planta 237, 705–715.Google Scholar
Knox, JP, Linstead, PJ, Peart, J, Cooper, C Roberts, K (1991) Developmentally regulated epitopes of cell surface arabinogalactan proteins and their relation to root tissue pattern formation. Plant J 1, 317–326.Google Scholar
Le Duigou, A Baley, C (2014) Coupled micromechanical analysis and life cycle assessment as an integrated tool for natural fibre composites development. J Clean Prod 83, 61–69.Google Scholar
Marcus, SE, Blake, AW, Benians, TAS, Lee, KJD, Poyser, C, Donaldson, L, Leroux, O, Rogowski, A, Petersen, HL, Boraston, A, Gilbert, HJ, Willats, WGT Knox, JP (2010) Restricted access of proteins to mannan polysaccharides in intact plant cell walls. Plant J 64, 191–203.Google Scholar
Marcus, SE, Verhertbruggen, Y, Herve, C, Ordaz-ortiz, JJ, Farkas, V, Pedersen, HL, Willats, WGT Knox, JP (2008) Pectic homogalacturonan masks abundant sets of xyloglucan epitopes in plant cell walls. BMC Plant Biol 8, 60.Google Scholar
Marrot, L, Lefeuvre, A, Pontoire, B, Bourmaud, A Baley, C (2013) Analysis of the hemp fiber mechanical properties and their scattering (Fedora 17). Ind Crops Prod 51, 317–327.Google Scholar
Martínez, ÁT, Rencoret, J, Marques, G, Gutierrez, A, Ibarra, D, Jimenez-Barbero, J del Río, JC (2008) Monolignol acylation and lignin structure in some non woody plants: A 2D NMR study. Phytochemistry 69, 2831–2843.Google Scholar
McCartney, L, Marcus, SE Knox, JP (2005) Monoclonal antibodies to plant cell wall xylans and arabinoxylans. J Histochem Cytochem 53, 543–546.Google Scholar
McDougall, GJ (1993) Isolate ion and partial characterisation of the non-cellulosic polysaccharides of flax fibre. Carbohydr Res 241, 227–236.Google Scholar
Mediavilla, V, Leupin, M Keller, A (2001) Influence of the growth stage of industrial hemp on the yield formation in relation to certain fibre quality traits. Ind Crops Prod 13, 49–56.Google Scholar
Nakagawa, K, Yoshinaga, A Takabe, K (2012) Anatomy and lignin distribution in reaction phloem fibres of several Japanese hardwoods. Ann Bot 110, 897–904.Google Scholar
Nanko, H (1979) Studies on the development cell wall structure of sclerenchymatous elements in the secondary phloem of woody dicotyledons, conifers. PhD Thesis. Kyoto University, Japan (in English).Google Scholar
Pettolino, FA, Hoogenraad, NJ, Ferguson, C, Bacic, A, Johnson, E Stone, BA (2001) A (1-4)-beta-mannan-specific monoclonal antibody and its use in the immunocytochemical location of galactomannans. Planta 214, 235–242.Google Scholar
Reis, D Vian, B (2004) Helicoidal pattern in secondary cell walls and possible role of xylans in their construction. C R Biol 327, 785–790.Google Scholar
Ruel, K, Burlat, V Joseleau, JP (1999) Relationship between ultrastructural topochemistry of lignin and wood properties. IAWA J 20, 203–211.Google Scholar
Ruel, K, Chabannes, M, Boudet, AM, Legrand, M Joseleau, JP (2001) Reassessment of quantitative changes in lignification of transgenic tobacco plants and their impact on cell wall assembly. Phytochem 57, 875–882.Google Scholar
Ruel, K, Faix, O Joseleau, JP (1994) New immunogold probes for studying the distribution of the different lignin types during plant cell wall biogenesis. J Trace Microprobe Tech 12, 247–265.Google Scholar
Sankari, HS (2000) Comparison of bast fibre yield and mechanical fibre properties of hemp (Cannabis sativa L.) cultivars. Ind Crops Prod 11, 73–84.Google Scholar
Schäfer, T Honermeier, B (2006) Effect of sowing date and plant density on the cell morphology of hemp (Cannabis sativa L.). Ind Crops Prod 23, 88–98.Google Scholar
Summerscales, J, Dissanayake, NPJ, Virk, AS Hall, W (2010) A review of bast fibres and their composites. Part 1—Fibres as reinforcements. Compos Part A 41, 1329–1335.Google Scholar
Thuault, A, Domenges, B, Hervas, I Gomina, M (2015) Investigation of the internal structure of flax fibre cell walls by transmission electron microscopy. Cellulose 22, 3521–3530.Google Scholar
Van der Werf, HMG, Van Geel, WCA, Van Gils, LJC Haverkort, AJ (1995b) Nitrogen fertilization and row width affect self-thinning and productivity of fibre hemp (Cannabis sativa L.). Field Crops Res 42, 27–37.Google Scholar
Van der Werf, HMG, Wijlhuizen, M Deschutter, JAA (1995a) Plant-density and self-thinning affect yield and quality of fiber hemp (Cannabis sativa L.). Field Crops Res 40, 153–164.Google Scholar
Vignon, M, Dupeyre, D Garcia-Jaldon, C (1995) Steam explosion of woody hemp chènevotte. Int J Biol Macromol 17, 395–404.Google Scholar
Vignon, M Garcia-Jaldon, C (1996) Structural features of the pectic polysaccharides isolated from retted hemp bast fibres. Carbohydr Res 296, 249–260.Google Scholar
Westerhuis, W, Amaducci, S, Struik, PC, Zatta, A, Van Dam, JEG Stomph, TJ (2009) Sowing density and harvest time affect fibre content in hemp (Cannabis sativa L.) through their effects on stem weight. Ann Appl Biol 155, 225–244.Google Scholar
Wiener, Y, Kovačič, V Dejlov, ÀP (2003) Differences between flax and hemp. Autex Res J 3, 58–63.Google Scholar