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Development and Evaluation of a Novel Topical Treatment for Acne with Azelaic Acid-Loaded Nanoparticles

Published online by Cambridge University Press:  15 May 2013

Catarina Pinto Reis ,
Ana Gomes ,
Patrícia Rijo ,
Sara Candeias ,
Pedro Pinto ,
Marina Baptista ,
Nuno Martinho  and
Lia Ascensão
Catarina Pinto Reis
Affiliation:
Laboratory of Nanoscience and Biomedical Nanotechnology (LNBN), CBIOS, Universidade Lusófona, Campo Grande 376, 1749-024 Lisboa, Portugal
Ana Gomes*
Affiliation:
Laboratory of Nanoscience and Biomedical Nanotechnology (LNBN), CBIOS, Universidade Lusófona, Campo Grande 376, 1749-024 Lisboa, Portugal
Patrícia Rijo
Affiliation:
Laboratory of Pharmacology and Therapeutics (LPT), CBIOS, Universidade Lusófona, Campo Grande 376, 1749-024 Lisboa, Portugal
Sara Candeias
Affiliation:
Laboratory of Nanoscience and Biomedical Nanotechnology (LNBN), CBIOS, Universidade Lusófona, Campo Grande 376, 1749-024 Lisboa, Portugal
Pedro Pinto
Affiliation:
Experimental Dermatology Unit (UDE), CBIOS, Universidade Lusófona, Campo Grande 376, 1749-024 Lisboa, Portugal
Marina Baptista
Affiliation:
Laboratory of Pharmacology and Therapeutics (LPT), CBIOS, Universidade Lusófona, Campo Grande 376, 1749-024 Lisboa, Portugal
Nuno Martinho
Affiliation:
Laboratory of Nanoscience and Biomedical Nanotechnology (LNBN), CBIOS, Universidade Lusófona, Campo Grande 376, 1749-024 Lisboa, Portugal
Lia Ascensão
Affiliation:
Departamento de Biologia Vegetal, IBB, Centro de Biotecnologia Vegetal, Faculdade de Ciências, Universidade de Lisboa, Lisboa 1749-016, Portugal
*
*Corresponding author. E-mail: [email protected]
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Abstract

Azelaic acid (AzA) is used in the treatment of acne. However, side effects and low compliance have been associated with several topical treatments with AzA. Nanotechnology presents a strategy that can overcome these problems. Polymeric nanoparticles can control drug release and targeting and reduce local drug toxicity. The aim of this study was to produce and evaluate an innovative topical treatment for acne with AzA-loaded poly-dl-lactide/glycolide copolymer nanoparticles. A soft white powder of nanoparticles was prepared. The mean size of loaded nanoparticles was <400 nm and zeta potential was negative. Spherical nanoparticles were observed by scanning electron microscopy. Encapsulation efficiency was around 80% and a strong interaction between the polymer and the drug was confirmed by differential scanning calorimetric analysis. In vitro drug release studies suggested a controlled and pulsatile release profile. System efficacy tests suggested similar results between the loaded nanoparticles and the nonencapsulated drug against the most common bacteria associated with acne. Cytotoxicity of AzA-loaded nanoparticles was concentration dependent, although not pronounced. The occluded patch test seemed to indicate that the formulation excipients were safe and thus AzA-loaded nanoparticles appear to be an efficient and safe treatment for acne.

Keywords

acne vulgarisazelaic acidtopical treatmentnanotechnologyefficacy and safetySEM
Type
Portuguese Society for Microscopy
Information
Microscopy and Microanalysis , Volume 19 , Issue 5 , October 2013 , pp. 1141 - 1150
Copyright
Copyright © Microscopy Society of America 2013 

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