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Cytotoxicity of Experimental Resin Composites on Mesenchymal Stem Cells Isolated from Two Oral Sources

Published online by Cambridge University Press:  09 September 2016

Alexandra Roman ,
Emöke Páll ,
Mărioara Moldovan ,
Darian Rusu ,
Olga Şoriţău ,
Dana Feştilă  and
Mihaela Lupşe
Alexandra Roman*
Affiliation:
Department of Periodontology, Iuliu Haţieganu University of Medicine and Pharmacy, 15 V. Babeş St., 400012 Cluj-Napoca, Romania
Emöke Páll
Affiliation:
Department of Veterinary Reproduction, Obstetrics and Gynecology, University of Agricultural Sciences and Veterinary Medicine, 3-5 Mănăştur St., 400372 Cluj-Napoca, Romania
Mărioara Moldovan
Affiliation:
Raluca Ripan Institute for Research in Chemistry, Babes-Bolyai University, 30 Fântânele St., 400294 Cluj-Napoca, Romania
Darian Rusu
Affiliation:
Periodontology Department, Victor Babes University of Medicine and Pharmacy, Bv. Revolutiei nr.9, 300014 Timisoara, Romania
Olga Şoriţău
Affiliation:
Laboratory of Tumor Biology and Radiobiology, Prof. Dr. Ion Chiricuţă Oncology Institute, 34-36 Republicii, 400015 Cluj-Napoca, Romania
Dana Feştilă
Affiliation:
Department of Orthodontics, Iuliu Haţieganu University of Medicine and Pharmacy, 33 Motilor St., 400001 Cluj-Napoca, Romania
Mihaela Lupşe
Affiliation:
Department of Infectious Diseases, Faculty of Medicine, Iuliu Haţieganu University of Medicine and Pharmacy, 23 Iuliu Moldovan St., 400349 Cluj-Napoca, Romania
*
*Corresponding author. [email protected]
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Abstract

Resin composite materials that are used to restore tooth cervical lesions associated with gingival recessions can hamper healing after root coverage surgeries. This study evaluates the in vitro cytotoxic effect of five resin composites (two commercial and three experimental) on oral mesenchymal stem cells (MSCs) and the persistence of stemness properties in high passage MSCs. Sorption and solubility tests were made for all materials. MSCs were isolated from re-entry palatal and periodontal granulation tissues and were characterized and cultured on composite discs. Cytotoxicity of the materials was evaluated by the Alamar Blue viability test, by Paul Karl Horan (PKH) labeling, and by immunocytochemical staining for actin. Water and saliva sorption and solubility data revealed that two of the experimental materials behaved comparable with the marketed resin composites. The Alamar Blue viability test shows that both cell lines grew well on composite discs that seemed to induce no apparent toxic effects. No signs of disruption of cytoskeleton organization was seen. Experimental resin composites can be recommended for further investigation for obtaining approval for use. The standard minimal criteria were fulfilled for high passage MSCs. Palatal tissue regains its regenerative properties in terms of MSC presence in the re-entry area after 6 months of healing.

Keywords

resin compositecytotoxicityleakagestem cells
Type
Biological Applications
Information
Microscopy and Microanalysis , Volume 22 , Issue 5 , October 2016 , pp. 1018 - 1033
Copyright
© Microscopy Society of America 2016 

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Footnotes

a

All the authors have equally contributed to the present study and can be regarded, therefore, as being main authors.

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