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Cytomegalovirus Capsid Structure and Tegument Binding

Published online by Cambridge University Press:  02 July 2020

A. C. Steven ,
W. Gibson ,
N. Cheng  and
B. L. Trus
A. C. Steven
Affiliation:
Laboratory of Structural Biology, NIAMS Engineering Laboratory, DCRT, National Institutes of Health, Bethesda, MD20892
W. Gibson
Affiliation:
Virology Laboratories, Johns Hopkins School of Medicine, Baltimore, MD21205
N. Cheng
Affiliation:
Laboratory of Structural Biology, NIAMS Engineering Laboratory, DCRT, National Institutes of Health, Bethesda, MD20892
B. L. Trus
Affiliation:
Laboratory of Structural Biology, NIAMS Engineering Laboratory, DCRT, National Institutes of Health, Bethesda, MD20892 Computational Bioscience and Engineering Laboratory, DCRT, National Institutes of Health, Bethesda, MD20892
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Herpesviruses form an extensive family of DNA viruses, in which three subfamilies called alpha-, beta- and gammaherpesviruses, respectively, are distinguished on the basis of biological properties. In terms of overall structure, all herpesviruses conform to a common plan: the virion consists of a thick-walled capsid, ∼ 1250 Å in diameter, containing the genome, surrounded by a complex layer of proteins called the tegument (a feature unique to herpesviruses), and a lipoprotein envelope. At a more detailed level, it has not been clear whether the three subfamilies are also differentiated structurally. The molecular architecture of alphaherpesvirus capsids has been studied by cryoelectron microscopy of herpes simplex virus 1, revealing its icosahedral (T=16) surface lattice and the locations of the four abundant shell proteins. To ascertain how closely these features are emulated in a betaherpesvirus, we have studied capsids of simian cytomegalovirus (SCMV) by similar methods. By comparing the structures of nuclear capsids and cytoplasmic capsids (which have bound some tegument proteins),

Type
Chambers and Channels: Functional Connections in Multiprotein Complexes Studied by Single Chambers and Channels
Information
Microscopy and Microanalysis , Volume 4 , Issue S2: Proceedings: Microscopy & Microanalysis '98, Microscopy Society of America 56th Annual Meeting, Microbeam Analysis Society 32nd Annual Meeting, Atlanta, Georgia July 12-16, 1998 , July 1998 , pp. 974 - 975
Copyright
Copyright © Microscopy Society of America

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References

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