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Cytochemical Labeling for Fungal and Host Components in Plant Tissues Inoculated with Fungal Wilt Pathogens

Published online by Cambridge University Press:  01 August 2004

G.B. Ouellette ,
R.P. Baayen ,
H. Chamberland ,
M. Simard ,
D. Rioux  and
P.M. Charest
G.B. Ouellette
Affiliation:
Natural Resources Canada, Canadian Forest Service, Laurentian Forestry Centre, 1055 du P.E.P.S., P.O. Box 3800, Sainte-Foy, Quebec G1V 4C7, Canada
R.P. Baayen
Affiliation:
Plant Protection Service, P.O. Box 9102, NL-6700 HC Wageningen, The Netherlands
H. Chamberland
Affiliation:
Département de phytologie, Pavillon C.-E.–Marchand, Université Laval, Quebec G1K 7P4, Canada
M. Simard
Affiliation:
Natural Resources Canada, Canadian Forest Service, Laurentian Forestry Centre, 1055 du P.E.P.S., P.O. Box 3800, Sainte-Foy, Quebec G1V 4C7, Canada
D. Rioux
Affiliation:
Natural Resources Canada, Canadian Forest Service, Laurentian Forestry Centre, 1055 du P.E.P.S., P.O. Box 3800, Sainte-Foy, Quebec G1V 4C7, Canada
P.M. Charest
Affiliation:
Département de phytologie, Pavillon C.-E.–Marchand, Université Laval, Quebec G1K 7P4, Canada
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Abstract

Antibodies to detect pectin in present investigations attached to distinct fibrils in vessel lumina. In carnation infected with an isolate of Fusarium oxysporum f.sp., labeling of pathogen cells also occurred; in a resistant cultivar (cv.), it was coincident with proximate pectin fibrils and linked to altered fungal walls, which was the opposite in the susceptible cv., indicating that hindrance of pathogen ability to degrade pectin may be related to resistance. Labeling of the fungus in culture was nil, except in media containing pectin, showing that pectin is not native to the pathogen. Labeling of fungal walls for cellulose in elm (inoculated with Ophiostoma novo-ulmi) and carnation also occurred, linked to adsorbed host wall components. The chitin probe often attached to dispersed matter, in vessel lumina, traceable to irregularly labeled fungal cells and host wall degradation products. With an anti-horseradish peroxidase probe, host and fungal walls were equally labeled, and with a glucosidase, differences of labeling between these walls were observed, depending on pH of the test solution. Fungal extracellular matter and filamentous structures, present in fungal walls, predominantly in another elm isolate (Phaeotheca dimorphospora), did not label with any of the probes used. However, in cultures of this fungus, extracellular material labeled, even at a distance from the colony margin, with an anti-fimbriae probe.

Keywords

colloidal gold-complexed probesfungal wilt pathogenspectinchitinperoxidasefungal extracellular matterfungal cell wallhost-pathogen relationships
Type
Biological Applications
Information
Microscopy and Microanalysis , Volume 10 , Issue 4 , August 2004 , pp. 449 - 461
Copyright
© 2004 Microscopy Society of America

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References

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