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Cryo-FIB Lamella Milling: A Comprehensive Technique to Prepare Samples of Both Plunge- and High-pressure Frozen-hydrated Specimens for in situ Studies.

Published online by Cambridge University Press:  01 August 2018

Miroslava Schaffer ,
Stefan Pfeffer ,
Stephan Kleindiek ,
Julia Mahamid ,
Michael Heymann ,
Andrew J. Smith ,
Tim Laugks ,
Benjamin D. Engel ,
Sahradha Albert  and
Wolfgang Baumeister
...Show all authors
Miroslava Schaffer
Affiliation:
Max Planck Institute of Biochemistry, Dep. of Molecular Structural Biology, Martinsried, Germany
Stefan Pfeffer
Affiliation:
Max Planck Institute of Biochemistry, Dep. of Molecular Structural Biology, Martinsried, Germany
Stephan Kleindiek
Affiliation:
Kleindiek Nanotechnik GmbH, Markwiesenstraße 55, Reutlingen, Germany
Julia Mahamid
Affiliation:
Max Planck Institute of Biochemistry, Dep. of Molecular Structural Biology, Martinsried, Germany Structural and Computational Biology Unit, European Molecular Biology Laboratory, Heidelberg
Michael Heymann
Affiliation:
Max Planck Institute of Biochemistry, Dep. of Cellular and Molecular Biophysics, Martinsried, Germany
Andrew J. Smith
Affiliation:
Kleindiek Nanotechnik GmbH, Markwiesenstraße 55, Reutlingen, Germany
Tim Laugks
Affiliation:
Max Planck Institute of Biochemistry, Dep. of Molecular Structural Biology, Martinsried, Germany
Benjamin D. Engel
Affiliation:
Max Planck Institute of Biochemistry, Dep. of Molecular Structural Biology, Martinsried, Germany
Sahradha Albert
Affiliation:
Max Planck Institute of Biochemistry, Dep. of Molecular Structural Biology, Martinsried, Germany
Wolfgang Baumeister
Affiliation:
Max Planck Institute of Biochemistry, Dep. of Molecular Structural Biology, Martinsried, Germany
Juergen M. Plitzko
Affiliation:
Max Planck Institute of Biochemistry, Dep. of Molecular Structural Biology, Martinsried, Germany

Abstract

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Type
Abstract
Information
Microscopy and Microanalysis , Volume 24 , Supplement S1: Proceedings of Microscopy & Microanalysis 2018 , August 2018 , pp. 820 - 821
Copyright
© Microscopy Society of America 2018 

References

[1] Schaffer, M, et al, J Struct Biol 197 2017) p. 73.Google Scholar
[2] Mahamid, J, et al, Science 351 2016) p. 969.Google Scholar
[3] Albert, S, et al, PNAS 114 2017) p. 13726.Google Scholar
[4] Bykov, YS, et al, eLife 6 2017 e32493.Google Scholar
[5] Schaffer, M, et al, Bio Protocol 5 2015 e1575.Google Scholar