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Cryo-Electron Microscopy Training at the Wadsworth Center

Published online by Cambridge University Press:  02 July 2020

Robert A. Grassucci  and
Joachim Frank
Robert A. Grassucci
Affiliation:
Howard Hughes Medical Institute at Health Research Inc., Wadsworth Center, Empire State Plaza, Albany, New York12201-0509
Joachim Frank
Affiliation:
Howard Hughes Medical Institute at Health Research Inc., Wadsworth Center, Empire State Plaza, Albany, New York12201-0509 Department of Biomedical Sciences, State University of New York at Albany, Empire State Plaza, Albany, New York12201-0509
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Extract

Training of students, postdocs and scientists in the techniques and instrumentation of Cryo-electron microscopy has many challenges. This is especially the case at the Wadsworth center where there are a variety of techniques as well as instruments to choose from, each of them having certain nuances that need to be recognized before they can be fully taken advantage of. There are also users with many levels of experience that need to be trained to use the equipment and techniques effectively. For this reason we have chosen to incorporate many of the protocols which are used into an internal web page. The web browser is a very effective tool for conveying detailed information on a variety of levels.

A novice user is asked to go through the EM training web page (Figure 1) and then select the Philips EM420 use link to get a feel for the steps that will be involved to set up and operate the microscope.

Type
Electron Cryomicroscopy of Macromolecules
Information
Microscopy and Microanalysis , Volume 6 , Issue S2: Proceedings: Microscopy & Microanalysis 2000, Microscopy Society of America 58th Annual Meeting, Microbeam Analysis Society 34th Annual Meeting, Microscopical Society of Canada/Societe de Microscopie de Canada 27th Annual Meeting, Philadelphia, Pennsylvania August 13-17, 2000 , August 2000 , pp. 278 - 279
Copyright
Copyright © Microscopy Society of America

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