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Cryo-Electron Microscopy of Aura Viruses

Published online by Cambridge University Press:  02 July 2020

W. Zhang ,
N. H. Olson ,
B. R. McKinney ,
R. J. Kuhn  and
T. S. Baker
W. Zhang
Affiliation:
Department of Biological Sciences, Purdue University, West Lafayette, IN47907-1392
N. H. Olson
Affiliation:
Department of Biological Sciences, Purdue University, West Lafayette, IN47907-1392
B. R. McKinney
Affiliation:
Department of Biological Sciences, Purdue University, West Lafayette, IN47907-1392
R. J. Kuhn
Affiliation:
Department of Biological Sciences, Purdue University, West Lafayette, IN47907-1392
T. S. Baker
Affiliation:
Department of Biological Sciences, Purdue University, West Lafayette, IN47907-1392
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Extract

Alphaviruses are a group of enveloped viruses in the Togaviridae family. Studies of several alphaviruses, including Ross River, Sindbis and Semliki Forest viruses, by cryo-electron microscopy (cryo-EM), three-dimensional (3D) image resconstruction and other techniques have illustrated that these spherical viruses have a T=4, multi-layered structure.

Aura virus, which is closely related to Sindbis, was first isolated in South America. Unlike the other alphaviruses, both genomic RNA (12kb, 49S) and subgenomic RNA(4.2kb, 26S) are encapsidated efficiently and form mature virions. Studies on negatively-stained virus particles demonstrated that there are two major size classes. The first contains particles of ∼72nm diameter, which are most similar to wild type virus, whereas the second class includes particles of ∼62nm in diameter. The 72nm particles are believed to have one copy of genomic RNA or one to three copies of subgenomic RNA, and a T=4 structure. The 62nm particles probably only have a single copy of subgenomic RNA and are presumed to be T=3 structures.

Type
Imaging of Macromolecular Complexes
Information
Microscopy and Microanalysis , Volume 4 , Issue S2: Proceedings: Microscopy & Microanalysis '98, Microscopy Society of America 56th Annual Meeting, Microbeam Analysis Society 32nd Annual Meeting, Atlanta, Georgia July 12-16, 1998 , July 1998 , pp. 946 - 947
Copyright
Copyright © Microscopy Society of America

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References

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