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Crio-fixation of Pachytene Cells

Published online by Cambridge University Press:  28 April 2020

R. Ortiz-Hernandez ,
G. H. Vázquez-Nin ,
O. Echeverría-Martínez ,
C. Höög  and
A. Hernández-Hernández
R. Ortiz-Hernandez
Affiliation:
Laboratorio de Microscopía Electrónica, Facultad de Ciencias, UNAM, México
G. H. Vázquez-Nin
Affiliation:
Laboratorio de Microscopía Electrónica, Facultad de Ciencias, UNAM, México
O. Echeverría-Martínez
Affiliation:
Laboratorio de Microscopía Electrónica, Facultad de Ciencias, UNAM, México
C. Höög
Affiliation:
Department of Cell and Molecular Biology, Karolinska Institutet, Stockholm
A. Hernández-Hernández*
Affiliation:
Laboratorio de Investigación en Patología Experimental, Hospital Infantil de México Federico Gómez, México
*
*Corresponding author:[email protected]

Abstract

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Genetic variability in organisms with sexual reproduction is produced by a complex mechanism of cell division of the germ line cells known as meiosis. During meiosis, homologous chromosomes pair and exchange genetic material (meiotic recombination), process that is essential to complete the meiotic division and to produce variability. Homologous chromosome pairing is mediated by the synaptonemal complex (SC). The SC is a proteinaceous structure composed of two lateral elements (LEs) and a central region (CR). Any defect in SC structure impairs meiotic recombination leading to blockage of the cell division process and infertility1. The SC has been observed since the introduction of the electron microscope (EM) in the biological field and it has been reported to be present in almost all the organisms with sexual reproduction, conserving a very similar structure and organisation along the different species2,3. The classic approach to study the SC structure is to chemically fixate the sexual tissues (gonads), dehydrate and embedded them in plastic resins that provide a support for sample sectioning and observation under the EM. However, chemical fixation followed by dehydration is well known to produce artefacts in the structure of many biological samples. Recently, we have combined fluorescence activated cell sorting of cells with SCs with high-pressure freezing and freeze- substitution and have found that the structure of the CR looks different from that observed in chemically fixated samples. These data have prompted us to analyse the organization of the CR components under cryo-processing.

Type
Abstract
Information
Microscopy and Microanalysis , Volume 26 , Supplement S1: Proceedings of 15th Interamerican Microscopy Congress CIASEM - SAMIC , March 2020 , pp. 43
Copyright
Copyright © Microscopy Society of America 2020

References

References:

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