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Correlative iPALM and Platinum Replica Electron Tomography to Highlight Single Molecules on Clathrin Endocytic Structures in 3D

Published online by Cambridge University Press:  23 September 2015

Kem Sochacki ,
Gleb Shtengel ,
Andrea Dickey ,
Harald Hess  and
Justin Taraska
Kem Sochacki
Affiliation:
National Heart Lung and Blood Institute, Bethesda, MD, USA.
Gleb Shtengel
Affiliation:
Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA.
Andrea Dickey
Affiliation:
National Heart Lung and Blood Institute, Bethesda, MD, USA.
Harald Hess
Affiliation:
Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA.
Justin Taraska
Affiliation:
National Heart Lung and Blood Institute, Bethesda, MD, USA.

Abstract

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Type
Abstract
Information
Microscopy and Microanalysis , Volume 21 , Supplement S3: Proceedings of Microscopy & Microanalysis 2015 , August 2015 , pp. 1497 - 1498
Copyright
Copyright © Microscopy Society of America 2015 

References

[1] McMahon, Harvey T. & Boucrot, Emmanuel, “Molecular mechanism and physiological functions of clathrin-mediated endocytosis.” Nature reviews Molecular cell biology 12.8 (2011): 517–533.Google Scholar
[2] Sochacki, Kem A, et al.. “Correlative super-resolution fluorescence and metal-replica transmission electron microscopy.” Nature methods 11.3 (2014): 305–308.Google Scholar
[3] Heuser, John. “Three-dimensional visualization of coated vesicle formation in fibroblasts.” The Journal of cell biology 84.3 (1980): 560–583.Google Scholar
[4] Shtengel, Gleb, et al. “Interferometric fluorescent super-resolution microscopy resolves 3D cellular ultrastructure.” Proceedings of the National Academy of Sciences 106.9 (2009): 3125–3130.Google Scholar