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Correlated 3D Light and Electron Microscopy of Large, Complex Structures: Analysis of Transverse Tubules in Heart Failure

Published online by Cambridge University Press:  02 July 2020

Maryann E. Martone ,
Andrea Thor ,
Stephen J. Young  and
Mark H. Ellisman.
Maryann E. Martone
Affiliation:
National Center for Microscopy and Imaging Research, Dept. of Neuroscience, San Diego, CA, 92093-0608.
Andrea Thor
Affiliation:
National Center for Microscopy and Imaging Research, Dept. of Neuroscience, San Diego, CA, 92093-0608.
Stephen J. Young
Affiliation:
National Center for Microscopy and Imaging Research, Dept. of Neuroscience, San Diego, CA, 92093-0608.
Mark H. Ellisman.
Affiliation:
National Center for Microscopy and Imaging Research, Dept. of Neuroscience, San Diego, CA, 92093-0608.
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Extract

Light microscopic imaging has experienced a renaissance in the past decade or so, as new techniques for high resolution 3D light microscopy have become readily available. Light microscopic (LM) analysis of cellular details is desirable in many cases because of the flexibility of staining protocols, the ease of specimen preparation and the relatively large sample size that can be obtained compared to electron microscopic (EM) analysis. Despite these advantages, many light microscopic investigations require additional analysis at the electron microscopic level to resolve fine structural features.

High voltage electron microscopy allows the use of relatively thick sections compared to conventional EM and provides the basis for excellent new methods to bridge the gap between microanatomical details revealed by LM and EM methods. When combined with electron tomography, investigators can derive accurate 3D data from these thicker specimens. Through the use of correlated light and electron microscopy, 3D reconstructions of large cellular or subcellular structures can be obtained with the confocal microscope,

Type
Unique Approaches in Imaging, Computation and Communication for Characterization of the 3D Cell & Organelles I
Information
Microscopy and Microanalysis , Volume 4 , Issue S2: Proceedings: Microscopy & Microanalysis '98, Microscopy Society of America 56th Annual Meeting, Microbeam Analysis Society 32nd Annual Meeting, Atlanta, Georgia July 12-16, 1998 , July 1998 , pp. 440 - 441
Copyright
Copyright © Microscopy Society of America

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References

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4. This work was supported by Grant In Aid #95-282 to MEM from the California Heart Association and RR04050 to MHE from the National Institute of Health.Google Scholar