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Consequences of a Fat Diet in the Distribution of Minerals within Pancreatic Tissues of Rats and Rabbits

Published online by Cambridge University Press:  09 October 2012

M. Dolores Ynsa ,
Ren Minquin ,
Reshmi Rajendran ,
Teresa Pinheiro  and
Frank Watt
M. Dolores Ynsa*
Affiliation:
Centro de Micro-Análisis de Materiales, Universidad Autonoma de Madrid, Campus de Cantoblanco, 28049 Madrid, Spain Dpto. Física Aplicada C-12, Universidad Autónoma de Madrid, 28049 Madrid, Spain Instituto de Ciencia de Materiales Nicolás Cabrera, Univ. Autónoma de Madrid, E-28049 Madrid, Spain
Ren Minquin
Affiliation:
Centre for Ion Beam Applications, Department of Physics, National University of Singapore, Singapore117542
Reshmi Rajendran
Affiliation:
Centre for Ion Beam Applications, Department of Physics, National University of Singapore, Singapore117542
Teresa Pinheiro
Affiliation:
Instituto Tecnológico e Nuclear & Centro de Física Nuclear, Fundação da Faculdade de Ciências, Universidade de Lisboa, Portugal
Frank Watt
Affiliation:
Centre for Ion Beam Applications, Department of Physics, National University of Singapore, Singapore117542
*
*Corresponding author. E-mail: [email protected]
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Abstract

The effects of plasma lipid overload on pancreatic islet function and on mineral imbalance are issues under debate. However, the outcomes may be biased by the different metabolisms of different species. This prospective study evaluated whether a high fat diet intake changed the distribution of physiologically relevant elements within pancreatic endocrine and exocrine tissues of Sprague Dawley rats and New Zealand White rabbits. Nuclear microscopy techniques provided images of the specimen density and structure as well as the elemental distributions and quantification of P, S, Cl, K, Ca, Fe, and Zn using unstained cryosections of pancreas. Our results indicate that pancreatic islets in normal rats and rabbits had lower tissue density and higher Ca, Fe, and Zn content compared to exocrine tissue, and that rabbit islets exhibit the highest Zn content (3,300 μg/g in rabbits versus 510 μg/g in rats). Fat diet intake resulted in large deposits of fat in the pancreas, which modified the density contrast of tissues and also resulted in a twofold decrease of Ca and Zn concentrations in islets of both rats and rabbits. This result indicates that a fat diet leads to a reduction in essential trace element concentrations in pancreas, which in turn may hamper endocrine function.

Keywords

zincironnuclear microscopyislets of Langerhansrabbitrat
Type
Biological Applications
Information
Microscopy and Microanalysis , Volume 18 , Issue 5 , October 2012 , pp. 1060 - 1066
Copyright
Copyright © Microscopy Society of America 2012

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