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Comparison of Ultrastructural Morphology of Tissue fixed in Formalin and Prefer™

Published online by Cambridge University Press:  02 July 2020

Steven D. Billings  and
Michael P. Goheen
Steven D. Billings
Affiliation:
University Hospital Indiana University Medical Center Indianapolis, Indiana
Michael P. Goheen
Affiliation:
University Hospital Indiana University Medical Center Indianapolis, Indiana
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Extract

Ultrastructural examination is sometimes necessary for diagnosis of surgical pathology material. Not uncommonly, tissue is not fixed in 3% glutaraldehyde prior to realizing that electron microscopy would be valuable or even necessary to render a diagnosis. It is well known that formalin can be an adequate fixative for ultrastructural evaluation of tissue. Increasingly, non-formalin based fixatives have become more popular with routine surgical pathology practice. Prefer™, a glyoxal-based fixative, has become a popular substitute for formalin in many institutions. We are not aware of any study that has compared ultrastructural morphology of tissue fixed in formalin to tissue fixed in a glyoxal-based fixative.

To address this question, we took sections of fresh tissue from three different tumors (metastatic melanoma involving a lymph node, squamous cell carcinoma of the lung, and mixed papillary serous and clear cell carcinoma of the ovary) and fixed a portion of each in 3% glutaraldehyde, 10% neutral buffered formalin, and Prefer™.

Type
Pathology
Information
Microscopy and Microanalysis , Volume 5 , Issue S2: Proceedings: Microscopy & Microanalysis '99, Microscopy Society of America 57th Annual Meeting, Microbeam Analysis Society 33rd Annual Meeting, Portland, Oregon August 1-5, 1999 , August 1999 , pp. 1158 - 1159
Copyright
Copyright © Microscopy Society of America

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References

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