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Comparative Assessment of Oral Mesenchymal Stem Cells Isolated from Healthy and Diseased Tissues

Published online by Cambridge University Press:  28 August 2015

Emöke Páll ,
Adrian Florea ,
Olga Soriţău ,
Mihai Cenariu ,
Adrian S. Petruţiu  and
Alexandra Roman
Emöke Páll
Affiliation:
Department of Reproduction, Obstetrics and Veterinary Gynecology, Faculty of Veterinary Medicine, University of Agricultural Sciences and Veterinary Medicine, 3-5 Mănăştur St., 400372 Cluj-Napoca, Romania Department of Periodontology, Faculty of Dental Medicine, “Iuliu Haţieganu” University of Medicine and Pharmacy, 15 V. Babeş St., 400012 Cluj-Napoca, Romania
Adrian Florea*
Affiliation:
Department of Cell and Molecular Biology, Faculty of Medicine, “Iuliu Haţieganu” University of Medicine and Pharmacy, 6 L. Pasteur St., 400349 Cluj-Napoca, Romania
Olga Soriţău
Affiliation:
Laboratory of Radiotherapy, Tumor and Radiobiology, Prof. Dr. “Ion Chiricuţă” Oncology Institute, 34-36 I. Creangă St., 400015 Cluj-Napoca, Romania
Mihai Cenariu
Affiliation:
Department of Reproduction, Obstetrics and Veterinary Gynecology, Faculty of Veterinary Medicine, University of Agricultural Sciences and Veterinary Medicine, 3-5 Mănăştur St., 400372 Cluj-Napoca, Romania
Adrian S. Petruţiu
Affiliation:
Department of Periodontology, Faculty of Dental Medicine, “Iuliu Haţieganu” University of Medicine and Pharmacy, 15 V. Babeş St., 400012 Cluj-Napoca, Romania
Alexandra Roman
Affiliation:
Department of Periodontology, Faculty of Dental Medicine, “Iuliu Haţieganu” University of Medicine and Pharmacy, 15 V. Babeş St., 400012 Cluj-Napoca, Romania
*
*Corresponding authors. [email protected]; [email protected]
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Abstract

The aim of the present study was to isolate human mesenchymal stem cells (MSCs) from palatal connective and periodontal granulation tissues and to comparatively evaluate their properties. MSCs were isolated using the explant culture method. Adherence to plastic, specific antigen makeup, multipotent differentiation potential, functionality, and ultrastructural characteristics were investigated. The frequency of colony-forming unit fibroblasts for palatal-derived mesenchymal stem cells (pMSCs) was significantly higher than that of granulation tissue-derived mesenchymal stem cells (gtMSCs). A significantly higher population doubling time and lower migration potential were recorded for gtMSCs than for pMSCs. Both cell lines were positive for CD105, CD73, CD90, CD44, and CD49f, and negative for CD34, CD45, and HLA-DR, but the level of expression was different. MSCs from both sources were relatively uniform in their ultrastructure. Generally, both cell lines possessed a large, irregular-shaped euchromatic nucleus, and cytoplasm rich in mitochondria, lysosomes, and endoplasmic reticulum. The periphery of the plasma membrane displayed many small filopodia. MSCs from both cell lines were successfully differentiated into osteogenic, adiopogenic, and chondrogenic lineages. Both healthy and diseased tissues may be considered as valuable sources of MSCs for regenerative medicine owing to the high acceptance and fewer complications during harvesting.

Keywords

mesenchymal stem cellspalategranulation tissuedifferentiationultrastructure
Type
Biological Applications
Information
Microscopy and Microanalysis , Volume 21 , Supplement 5 , October 2015 , pp. 1249 - 1263
Copyright
© Microscopy Society of America 2015 

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