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Choosing a Suitable Ultramicrotome, Associated Equipment and the Proper Laboratory Environment for Operation of the System

Published online by Cambridge University Press:  02 July 2020

John J. Bozzola*
Affiliation:
Center for Electron Microscopy, Southern Illinois University, Carbondale, IL, 62901
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Extract

The selection of an ultramicrotome should always begin with an evaluation of the immediate as well as the long-term needs of the laboratory. If present projects require ultramicrotomy on a regular basis (e.g., more than 5-10 specimens per week) and if this demand will probably continue for more than one year, the acquisition of an ultramicrotome is justified. An intensive, short-term project or an occasional need with no clear indication of a future requirement for ultramicrotomy could best be accommodated by either leasing an ultramicrotome or contracting the work to an individual or corporation. On the other hand, several laboratories with modest needs may wish to pool resources and purchase a shared instrument capable of fulfilling the requirements of each lab.

Since new ultramicrotomes cost $20k and up, one may be compelled to acquire a used instrument. Depending upon the age and condition, a used ultramicrotome may be purchased at a cost of $2-15k.

Type
Instrumentation: How to Choose it and Use it
Copyright
Copyright © Microscopy Society of America

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References

Reid, N. and Beesley, J.E.. 1991. Sectioning and cryosectioning for electron microscopy. Vol. 13: Practical Methods in Electron Microscopy. A.M. Glauert, ed. (Elsevier, New York).Google Scholar