Hostname: page-component-586b7cd67f-dsjbd Total loading time: 0 Render date: 2024-12-01T04:06:17.749Z Has data issue: false hasContentIssue false
  • English
  • Français

Characterization of Titanium Alloy Particulates to Study the in Vitro Release of Inflammatory Mediators by Human Peripheral Blood Monocytes.

Published online by Cambridge University Press:  02 July 2020

B.J. Darien ,
P. Sims ,
T. Robinson ,
P. Manley  and
R. Albrecht
B.J. Darien
Affiliation:
Departments of Medical, University of Wisconsin, Madison, WI53706-1102
P. Sims
Affiliation:
Department of Animal Health and Biomedical Science, School of Agriculture and Life Science, University of Wisconsin, Madison, WI53706-1102
T. Robinson
Affiliation:
Surgical Sciences, School of Veterinary Medicine, University of Wisconsin, Madison, WI53706-1102
P. Manley
Affiliation:
Surgical Sciences, School of Veterinary Medicine, University of Wisconsin, Madison, WI53706-1102
R. Albrecht
Affiliation:
Department of Animal Health and Biomedical Science, School of Agriculture and Life Science, University of Wisconsin, Madison, WI53706-1102
Get access

Extract

Particulate wear debris found around joint arthroplasties has been linked to the premature failure of artificial joints. Cytokines and eicosanoids released by macrophages are known mediators of inflammation and osteolysis. Previous in vitro studies of wear debris-mediated inflammation have relied upon poorly characterized particulates and peripheral blood cell fractions of mixed cellular composition. The objective of this study was to utilize titanium particulates that closely match wear debris found around joint arthroplasties and to study their effects on the biosynthesis of inflammatory mediators by isolated monocytes.

To mimic wear debris that surrounds artificial joints, titanium alloy particulates (1-3 μm in diameter) were obtained. A submicron fraction of these particulates was isolated by differential sedimentation. The morphology, size and concentration of the resulting population was characterized utilizing scanning electron microscopy. Titanium particulate size was estimated from photomicrographs of calibrated latex microspheres mixed with titanium particles (Figure 1). A known volume of titanium particulates in suspension was applied to a carbon planchette and allowed to dry.

Type
Biomaterials
Information
Microscopy and Microanalysis , Volume 4 , Issue S2: Proceedings: Microscopy & Microanalysis '98, Microscopy Society of America 56th Annual Meeting, Microbeam Analysis Society 32nd Annual Meeting, Atlanta, Georgia July 12-16, 1998 , July 1998 , pp. 940 - 941
Copyright
Copyright © Microscopy Society of America

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

References:

1.Goldring, S.R., et al., Journal of Bone and Joint Surgery 65(1983)575.CrossRefGoogle Scholar
2.Giant, T.T., et al., Journal of Bone and Mineral Research 8(1993)1071.Google Scholar
3.Rogers, S.D., et al., Journal of Orthopaedic Research 11(1993)856.CrossRefGoogle Scholar
4.Maloney, W.J., et al., The Journal of Bone and Joint Surgery 77(1995)1301.CrossRefGoogle Scholar