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Characterization of Keratin Pearls in Oral Squamous Cell Carcinoma Using FT-IR Microspectroscopy

Published online by Cambridge University Press:  02 July 2020

C.P. Schultz ,
K.-Z. Liu ,
P.D. Kerr  and
H.H. Mantsch
C.P. Schultz
Affiliation:
Institute for Biodiagnostics, National Research Council Canada, Winnipeg, MBR3C 1Y6
K.-Z. Liu
Affiliation:
Institute for Biodiagnostics, National Research Council Canada, Winnipeg, MBR3C 1Y6
P.D. Kerr
Affiliation:
Department of Otolaryngology, Health Sciences Center, Winnipeg, MBR3A 1R9
H.H. Mantsch
Affiliation:
Institute for Biodiagnostics, National Research Council Canada, Winnipeg, MBR3C 1Y6
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Extract

Oral squamous cell carcinoma (SCC) positive biopsies from patients who underwent excision procedures by micrographical surgery were analyzed by FT-IR microspectroscopy in order to examine a specific fine structure of abnormal epithelial growth - the so-called keratin pearls. Taken from the oral cavity of 15 SCC positive patients, all biopsies indicated poorly or well-differentiated cancer containing smaller and larger pearls. In some cases only a few pearls were present; in others many of various sizes.

After surgery the biopsies were rapidly frozen in liquid nitrogen and stored at -80°C before thin sectioning the tissue for infrared measurements. The frozen tissue was then sliced into 8 μm thin sections and dried on an infrared-transparent Bal2window. For comparison one slice of tissue was always used for hematoxylin and eosin staining by drying it on a standard microscope slide. This procedure creates a series of thin sections on BaF2 and glass following an alternate pattern of one 8 μm section for IR microspectroscopy and the next 8 μm one for microscopy.

Type
Optical Microanalysis
Information
Microscopy and Microanalysis , Volume 3 , Issue S2: Proceedings: Microscopy & Microanalysis '97, Microscopy Society of America 55th Annual Meeting, Microbeam Analysis Society 31st Annual Meeting, Histochemical Society 48th Annual Meeting, Cleveland, Ohio, August 10-14, 1997 , August 1997 , pp. 833 - 834
Copyright
Copyright © Microscopy Society of America 1997

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References

1.Choo, L.-P.et al., Biophys.J., 71 (1996) 1672.10.1016/S0006-3495(96)79411-0CrossRefGoogle Scholar
2.Schultz, C.P.et al., Leukemia Res. 20 (1996) 649.10.1016/0145-2126(96)00019-7CrossRefGoogle Scholar